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000079781 005__ 20230921135427.0
000079781 0247_ $$2doi$$a10.3389/fvets.2019.00175
000079781 0248_ $$2sideral$$a112664
000079781 037__ $$aART-2019-112664
000079781 041__ $$aeng
000079781 100__ $$aGuzmán-Verri, Caterina
000079781 245__ $$aGenetic and Phenotypic Characterization of the Etiological Agent of Canine Orchiepididymitis Smooth Brucella sp. BCCN84.3
000079781 260__ $$c2019
000079781 5060_ $$aAccess copy available to the general public$$fUnrestricted
000079781 5203_ $$aMembers of the genus Brucella cluster in two phylogenetic groups: classical and non-classical species. The former group is composed of Brucella species that cause disease in mammals, including humans. A Brucella species, labeled as Brucella sp. BCCN84.3, was isolated from the testes of a Saint Bernard dog suffering orchiepididymitis, in Costa Rica. Following standard microbiological methods, the bacterium was first defined as "Brucella melitensis biovar 2." Further molecular typing, identified the strain as an atypical "Brucella suis." Distinctive Brucella sp. BCCN84.3 markers, absent in other Brucella species and strains, were revealed by fatty acid methyl ester analysis, high resolution melting PCR and omp25 and omp2a/omp2b gene diversity. Analysis of multiple loci variable number of tandem repeats and whole genome sequencing demonstrated that this isolate was different from the currently described Brucella species. The smooth Brucella sp. BCCN84.3 clusters together with the classical Brucella Glade and displays all the genes required for virulence. Brucella sp. BCCN84.3 is a species nova taxonomical entity displaying pathogenicity; therefore, relevant for differential diagnoses in the context of brucellosis. Considering the debate on the Brucella species concept, there is a need to describe the extant taxonomical entities of these pathogens in order to understand the dispersion and evolution.
000079781 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000079781 590__ $$a2.245$$b2019
000079781 591__ $$aVETERINARY SCIENCES$$b19 / 141 = 0.135$$c2019$$dQ1$$eT1
000079781 592__ $$a0.859$$b2019
000079781 593__ $$aVeterinary (miscellaneous)$$c2019$$dQ1
000079781 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000079781 700__ $$aSuárez-Esquivel, Marcela
000079781 700__ $$aRuíz-Villalobos, Nazareth
000079781 700__ $$aZygmunt, Michel, S.
000079781 700__ $$aGonnet, Mathieu
000079781 700__ $$aCampos, Elena
000079781 700__ $$aVíquez-Ruiz, Eunice
000079781 700__ $$aChacón-Díaz, Carlos
000079781 700__ $$aAragón-Aranda, Beatriz
000079781 700__ $$aConde-Álvarez, Raquel
000079781 700__ $$aMoriyón, Ignacio
000079781 700__ $$aBlasco, José, María
000079781 700__ $$0(orcid)0000-0002-7970-4929$$aMuñoz, Pilar, M.
000079781 700__ $$aBaker, Kate, S.
000079781 700__ $$aThomson, Nicholas, R.
000079781 700__ $$aCloeckaert, Axel
000079781 700__ $$aMoreno, Edgardo
000079781 773__ $$g6, 175 (2019), [13 pp]$$pFront. vet. sci.$$tFrontiers in Veterinary Science$$x2297-1769
000079781 8564_ $$s1823843$$uhttps://zaguan.unizar.es/record/79781/files/texto_completo.pdf$$yVersión publicada
000079781 8564_ $$s11549$$uhttps://zaguan.unizar.es/record/79781/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
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000079781 951__ $$a2023-09-21-13:29:29
000079781 980__ $$aARTICLE