000078862 001__ 78862
000078862 005__ 20190412155739.0
000078862 0247_ $$2doi$$a10.1371/journal.pone.0212136
000078862 0248_ $$2sideral$$a111236
000078862 037__ $$aART-2019-111236
000078862 041__ $$aeng
000078862 100__ $$aAlcolea, P.J.
000078862 245__ $$aIL12 p35 and p40 subunit genes administered as pPAL plasmid constructs do not improve protection of pPAL-LACK vaccine against canine leishmaniasis
000078862 260__ $$c2019
000078862 5060_ $$aAccess copy available to the general public$$fUnrestricted
000078862 5203_ $$aLeishmania infantum causes zoonotic visceral leishmaniasis (ZVL) in the Mediterranean basin and South America. The parasite has been shown to co-infect HIV patients and an outbreak in central Spain was reported in the last decade. Therfore, ZVL is a public health problem, dogs being the parasite’s reservoir. We have developed a DNA vaccine based on the L. infantum activated protein kinase A receptor (LACK) using different plasmid vectors and vaccinia virus strains as vehicles. Recently, we have generated an antibiotic resistance marker-free plasmid vector called pPAL. Homologous pPAL-LACK prime-boost vaccination protects Beagle dogs as well as a heterologous plasmid-virus regime. For both reasons, pPAL improves safety. IL12 was described to trigger Th1 response through IFN-¿ production in infected dogs, being a good candidate for cytokine therapy in conventional treatment-unresponsive dogs. Herein, we report a complete protection study in dogs through inoculation of genes encoding for the p35 and p40 subunits which compose canine IL12 in combination with the LACK gene. A homologous plasmid-plasmid regime using independent pPAL constructs for each gene was inoculated in a 15-day interval. The infectious challenge using L. infantum promastigotes was successful. The outcome was pPAL-LACK vaccine protection suppression by IL12 administration. The important implications of this finding are discussed in the manuscript.
000078862 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000078862 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000078862 700__ $$aAlonso, A.
000078862 700__ $$0(orcid)0000-0002-8282-5975$$aEsteban, A.
000078862 700__ $$aPeris, P.$$uUniversidad de Zaragoza
000078862 700__ $$0(orcid)0000-0002-2218-5072$$aCortés, A.$$uUniversidad de Zaragoza
000078862 700__ $$0(orcid)0000-0002-2048-4749$$aCastillo, J.A.$$uUniversidad de Zaragoza
000078862 700__ $$aLarraga, V.
000078862 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000078862 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000078862 773__ $$g14, 2 (2019), e0212136[18 pp]$$pPLoS One$$tPLoS ONE$$x1932-6203
000078862 8564_ $$s475928$$uhttp://zaguan.unizar.es/record/78862/files/texto_completo.pdf$$yVersión publicada
000078862 8564_ $$s91848$$uhttp://zaguan.unizar.es/record/78862/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000078862 909CO $$ooai:zaguan.unizar.es:78862$$particulos$$pdriver
000078862 951__ $$a2019-04-12-14:36:41
000078862 980__ $$aARTICLE