000070933 001__ 70933
000070933 005__ 20211008114732.0
000070933 0247_ $$2doi$$a10.1371/journal.pgen.1007282
000070933 0248_ $$2sideral$$a106521
000070933 037__ $$aART-2018-106521
000070933 041__ $$aeng
000070933 100__ $$0(orcid)0000-0001-8841-6593$$aGonzalo-Asensio, J.$$uUniversidad de Zaragoza
000070933 245__ $$aNew insights into the transposition mechanisms of IS6110 and its dynamic distribution between Mycobacterium tuberculosis Complex lineages
000070933 260__ $$c2018
000070933 5060_ $$aAccess copy available to the general public$$fUnrestricted
000070933 5203_ $$aThe insertion Sequence IS6110, only present in the pathogens of the Mycobacterium tuberculosis Complex (MTBC), has been the gold-standard epidemiological marker for TB for more than 25 years, but biological implications of IS6110 transposition during MTBC adaptation to humans remain elusive. By studying 2, 236 clinical isolates typed by IS6110-RFLP and covering the MTBC, we remarked a lineage-specific content of IS6110 being higher in modern globally distributed strains. Once observed the IS6110 distribution in the MTBC, we selected representative isolates and found a correlation between the normalized expression of IS6110 and its abundance in MTBC chromosomes. We also studied the molecular regulation of IS6110 transposition and we found a synergistic action of two post-transcriptional mechanisms: a -1 ribosomal frameshift and a RNA pseudoknot which interferes translation. The construction of a transcriptionally active transposase resulted in 20-fold increase of the transposition frequency. Finally, we examined transposition in M. bovis and M. tuberculosis during laboratory starvation and in a mouse infection model of TB. Our results shown a higher transposition in M. tuberculosis, that preferably happens during TB infection in mice and after one year of laboratory culture, suggesting that IS6110 transposition is dynamically adapted to the host and to adverse growth conditions.
000070933 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/BIO2014-52580P$$9info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI15-0317$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 643381-TBVAC2020$$9info:eu-repo/grantAgreement/EC/H2020/643381/EU/TBVAC2020; Advancing novel and promising TB vaccine candidates from discovery to preclinical and early clinical development/TBVAC2020$$9info:eu-repo/grantAgreement/ES/DGA/FSE
000070933 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000070933 590__ $$a5.224$$b2018
000070933 591__ $$aGENETICS & HEREDITY$$b23 / 174 = 0.132$$c2018$$dQ1$$eT1
000070933 592__ $$a4.001$$b2018
000070933 593__ $$aCancer Research$$c2018$$dQ1
000070933 593__ $$aEcology, Evolution, Behavior and Systematics$$c2018$$dQ1
000070933 593__ $$aMolecular Biology$$c2018$$dQ1
000070933 593__ $$aGenetics (clinical)$$c2018$$dQ1
000070933 593__ $$aGenetics$$c2018$$dQ1
000070933 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000070933 700__ $$0(orcid)0000-0001-5407-7271$$aPérez, I.$$uUniversidad de Zaragoza
000070933 700__ $$0(orcid)0000-0001-7897-9173$$aAguiló, N.$$uUniversidad de Zaragoza
000070933 700__ $$0(orcid)0000-0001-7866-2803$$aUranga, S.$$uUniversidad de Zaragoza
000070933 700__ $$0(orcid)0000-0003-4409-1437$$aPicó, A.$$uUniversidad de Zaragoza
000070933 700__ $$aLampreave, C.
000070933 700__ $$0(orcid)0000-0002-3312-7759$$aCebollada, A.
000070933 700__ $$0(orcid)0000-0003-1027-7583$$aOtal, I.$$uUniversidad de Zaragoza
000070933 700__ $$aSamper, S.
000070933 700__ $$0(orcid)0000-0003-2993-5478$$aMartín, C.$$uUniversidad de Zaragoza
000070933 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Microbiología
000070933 7102_ $$11008$$2X$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cProy. investigación HQA
000070933 773__ $$g14, 4 (2018), e1007282[23 pp]$$pPLoS Genet.$$tPLoS Genetics$$x1553-7390
000070933 8564_ $$s697816$$uhttps://zaguan.unizar.es/record/70933/files/texto_completo.pdf$$yVersión publicada
000070933 8564_ $$s103905$$uhttps://zaguan.unizar.es/record/70933/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000070933 909CO $$ooai:zaguan.unizar.es:70933$$particulos$$pdriver
000070933 951__ $$a2021-10-08-11:37:30
000070933 980__ $$aARTICLE