000070912 001__ 70912
000070912 005__ 20190709135532.0
000070912 0247_ $$2doi$$a10.1016/j.smallrumres.2017.03.011
000070912 0248_ $$2sideral$$a98577
000070912 037__ $$aART-2017-98577
000070912 041__ $$aeng
000070912 100__ $$aPérez-Laguna, V.
000070912 245__ $$aDaylight photodynamic therapy using methylene blue to treat sheep with dermatophytosis caused by Arthroderma vanbreuseghemii
000070912 260__ $$c2017
000070912 5060_ $$aAccess copy available to the general public$$fUnrestricted
000070912 5203_ $$aArthroderma vanbreuseghemii has been identified molecularly as the causative agent of dermatophytosis in a flock of sheep. It is necessary to explore new treatment alternatives because antifungals are not approved for use on small ruminant animals in the European Union. Antimicrobial photodynamic therapy (aPDT) has been shown to be effective for the treatment of dermatophytosis in humans. It is based on the application of a photosensitizer such as methylene blue (MB) that is activated by visible light to generate reactive oxygen species that are cytotoxic to cells. The use of daylight to perform aPDT (aDL-PDT) avoids the requirement of specific equipment because it uses sunlight to activate the photosensitizer. The aim of our study is to determine the efficacy of aDL-PDT using a 1% MB solution to treat dermatophytosis caused by A. vanbreuseghemii in ewes. Two different topical protocols (1% MB solution spray applications once or twice a week) were assayed in two groups of five infected animals. Twenty-five infected sheep were untreated. All the sheep were exposed to sunlight every day for an approximate duration of 10 h for a total of four weeks. At the end of the study, all the animals treated with aDL-PDT showed the same clinical response to both protocols. In contrast, the animals exposed only to sunlight required an additional two to four weeks before their infections resolved. Conclusion: aDL-PDT with 1% MB solution demonstrates efficacy, safety and efficiency in the treatment of dermatophytosis in sheep.
000070912 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/CTQ2013-48767-C3-2-R
000070912 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000070912 590__ $$a0.974$$b2017
000070912 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b31 / 59 = 0.525$$c2017$$dQ3$$eT2
000070912 592__ $$a0.485$$b2017
000070912 593__ $$aFood Animals$$c2017$$dQ2
000070912 593__ $$aAnimal Science and Zoology$$c2017$$dQ2
000070912 655_4 $$ainfo:eu-repo/semantics/conferenceObject$$vinfo:eu-repo/semantics/acceptedVersion
000070912 700__ $$0(orcid)0000-0001-7294-245X$$aRezusta, A.$$uUniversidad de Zaragoza
000070912 700__ $$0(orcid)0000-0002-2724-2785$$aRamos, J. J.$$uUniversidad de Zaragoza
000070912 700__ $$0(orcid)0000-0003-0042-8800$$aFerrer, L. M.$$uUniversidad de Zaragoza
000070912 700__ $$aGené, J.
000070912 700__ $$0(orcid)0000-0003-3570-5791$$aRevillo, M. J.
000070912 700__ $$aGilaberte, Y.
000070912 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Microbiología
000070912 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000070912 773__ $$g150 (2017), 97-101$$pSmall rumin. res.$$tSmall Ruminant Research$$x0921-4488
000070912 8564_ $$s715538$$uhttps://zaguan.unizar.es/record/70912/files/texto_completo.pdf$$yPostprint
000070912 8564_ $$s47677$$uhttps://zaguan.unizar.es/record/70912/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
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000070912 951__ $$a2019-07-09-12:03:03
000070912 980__ $$aARTICLE