000070706 001__ 70706
000070706 005__ 20190709135655.0
000070706 0247_ $$2doi$$a10.1038/s41467-017-02006-0
000070706 0248_ $$2sideral$$a104302
000070706 037__ $$aART-2017-104302
000070706 041__ $$aeng
000070706 100__ $$0(orcid)0000-0002-1924-334X$$aRivas, M.D.L.$$uUniversidad de Zaragoza
000070706 245__ $$aThe interdomain flexible linker of the polypeptide GalNAc transferases dictates their long-range glycosylation preferences
000070706 260__ $$c2017
000070706 5060_ $$aAccess copy available to the general public$$fUnrestricted
000070706 5203_ $$aThe polypeptide GalNAc-transferases (GalNAc-Ts), that initiate mucin-type O-glycosylation, consist of a catalytic and a lectin domain connected by a flexible linker. In addition to recognizing polypeptide sequence, the GalNAc-Ts exhibit unique long-range N- A nd/or C-terminal prior glycosylation (GalNAc-O-Ser/Thr) preferences modulated by the lectin domain. Here we report studies on GalNAc-T4 that reveal the origins of its unique N-terminal long-range glycopeptide specificity, which is the opposite of GalNAc-T2. The GalNAc-T4 structure bound to a monoglycopeptide shows that the GalNAc-binding site of its lectin domain is rotated relative to the homologous GalNAc-T2 structure, explaining their different long-range preferences. Kinetics and molecular dynamics simulations on several GalNAc-T2 flexible linker constructs show altered remote prior glycosylation preferences, confirming that the flexible linker dictates the rotation of the lectin domain, thus modulating the GalNAc-Ts'' long-range preferences. This work for the first time provides the structural basis for the different remote prior glycosylation preferences of the GalNAc-Ts.
000070706 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-70524-R$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2016-75633-P$$9info:eu-repo/grantAgreement/ES/MICINN/RYC-2013-14706$$9info:eu-repo/grantAgreement/ES/MICINN/CTQ2015-67727-R$$9info:eu-repo/grantAgreement/ES/MICINN/CTQ2013-44367-C2-2-P$$9info:eu-repo/grantAgreement/EC/FP7/609409/EU/Supporting transnational mobility within the European life sciences by cofunding of the EMBO Fellowship Programme/LTFCOFUND2013$$9info:eu-repo/grantAgreement/EC/FP7/283570/EU/Transnational access and enhancement of integrated Biological Structure determination at synchrotron X-ray radiation facilities/BIOSTRUCT-X$$9info:eu-repo/grantAgreement/ES/DGA/B89
000070706 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000070706 590__ $$a12.353$$b2017
000070706 591__ $$aMULTIDISCIPLINARY SCIENCES$$b3 / 64 = 0.047$$c2017$$dQ1$$eT1
000070706 592__ $$a6.582$$b2017
000070706 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2017$$dQ1
000070706 593__ $$aPhysics and Astronomy (miscellaneous)$$c2017$$dQ1
000070706 593__ $$aChemistry (miscellaneous)$$c2017$$dQ1
000070706 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000070706 700__ $$aLira-Navarrete, E.
000070706 700__ $$aDaniel, E.J.P.
000070706 700__ $$aCompanõn, I.
000070706 700__ $$aCoelho, H.
000070706 700__ $$aDiniz, A.
000070706 700__ $$aJiménez-Barbero, J.
000070706 700__ $$aPeregrina, J.M.
000070706 700__ $$aClausen, H.
000070706 700__ $$aCorzana, F.
000070706 700__ $$aMarcelo, F.
000070706 700__ $$aJiménez-Osés, G.
000070706 700__ $$aGerken, T.A.
000070706 700__ $$0(orcid)0000-0002-3122-9401$$aHurtado-Guerrero, R.$$uUniversidad de Zaragoza
000070706 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000070706 773__ $$g8, 1 (2017), 1959 [11 pp]$$pNATURE COMMUNICATIONS$$tNature Communications$$x2041-1723
000070706 8564_ $$s1673503$$uhttps://zaguan.unizar.es/record/70706/files/texto_completo.pdf$$yVersión publicada
000070706 8564_ $$s80274$$uhttps://zaguan.unizar.es/record/70706/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000070706 909CO $$ooai:zaguan.unizar.es:70706$$particulos$$pdriver
000070706 951__ $$a2019-07-09-12:45:50
000070706 980__ $$aARTICLE