000070639 001__ 70639
000070639 005__ 20211216131131.0
000070639 0247_ $$2doi$$a10.1088/2050-6120/4/4/042005
000070639 0248_ $$2sideral$$a106012
000070639 037__ $$aART-2016-106012
000070639 041__ $$aeng
000070639 100__ $$0(orcid)0000-0002-8973-5104$$aGalban, J.$$uUniversidad de Zaragoza
000070639 245__ $$aThe intrinsic fluorescence of FAD and its application in analytical chemistry: a review
000070639 260__ $$c2016
000070639 5060_ $$aAccess copy available to the general public$$fUnrestricted
000070639 5203_ $$aThis review (with 106 references) mainly deals with the analytical applications of flavin-adenine dinucleotide (FAD) fluorescence. In the first section, the spectroscopic properties of this compound are reviewed at the light of his different acid-base, oxidation and structural forms; the chemical and spectroscopic properties of flavin mononucleotide (FMN) and other flavins will be also briefly discussed. The second section discusses how the properties of FAD fluorescence changes in flavoenzymes (FvEs), again considering the different chemical and structural forms; the glucose oxidase (GOx) and the choline oxidase (ChOx) cases will be commented. Since almost certainly the most reported analytical application of FAD fluorescence is as an auto-indicator in enzymatic methods catalysed by FvE oxidoreductases, it is important to know how the concentrations of the different forms of FAD changes along the reaction and, consequently, the fluorescence and the analytical signals. An approach to do this will be presented in section 3. The fourth part of the paper compiles the analytical applications which have been reported until now based in these fluorescence properties. Finally, some suggestions about tentative future research are also given.
000070639 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E74$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2012-34774
000070639 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000070639 590__ $$a2.656$$b2016
000070639 591__ $$aCHEMISTRY, PHYSICAL$$b66 / 145 = 0.455$$c2016$$dQ2$$eT2
000070639 591__ $$aCHEMISTRY, ANALYTICAL$$b27 / 76 = 0.355$$c2016$$dQ2$$eT2
000070639 592__ $$a0.895$$b2016
000070639 593__ $$aAtomic and Molecular Physics, and Optics$$c2016$$dQ1
000070639 593__ $$aInstrumentation$$c2016$$dQ1
000070639 593__ $$aMaterials Science (miscellaneous)$$c2016$$dQ1
000070639 593__ $$aSpectroscopy$$c2016$$dQ2
000070639 655_4 $$ainfo:eu-repo/semantics/review$$vinfo:eu-repo/semantics/publishedVersion
000070639 700__ $$0(orcid)0000-0002-3906-4576$$aSanz-Vicente, I.$$uUniversidad de Zaragoza
000070639 700__ $$aNavarro, J.$$uUniversidad de Zaragoza
000070639 700__ $$0(orcid)0000-0002-7902-6005$$ade Marcos, S.$$uUniversidad de Zaragoza
000070639 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000070639 773__ $$g4, 4 (2016), 042005 [19 pp]$$pMethods appl. fluoresc.$$tMethods and Applications in Fluorescence$$x2050-6120
000070639 8564_ $$s1364004$$uhttps://zaguan.unizar.es/record/70639/files/texto_completo.pdf$$yVersión publicada
000070639 8564_ $$s106184$$uhttps://zaguan.unizar.es/record/70639/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
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000070639 951__ $$a2021-12-16-13:01:51
000070639 980__ $$aARTICLE