000070591 001__ 70591
000070591 005__ 20200221144300.0
000070591 0247_ $$2doi$$a10.1016/j.exer.2015.10.012
000070591 0248_ $$2sideral$$a93944
000070591 037__ $$aART-2016-93944
000070591 041__ $$aeng
000070591 100__ $$0(orcid)0000-0003-0349-9997$$aPinilla, Isabel$$uUniversidad de Zaragoza
000070591 245__ $$aLong time remodeling during retinal degeneration evaluated by optical coherence tomography, immunocytochemistry and fundus autofluorescence
000070591 260__ $$c2016
000070591 5060_ $$aAccess copy available to the general public$$fUnrestricted
000070591 5203_ $$aPurpose: To characterize the relationship between fundus autofluorescence (FAF), Optical Coherence Tomography (OCT) and immuno histo chemistry (IHC) over the course of chronic retinal degeneration in the P23H rat. Methods: Homozygous albino P23H rats, SpragueeDawley (SD) rats as controls and pigmented Long Evans (LE) rats were used. A Spectralis HRA OCT system was used for scanning laser ophthalmoscopy
(SLO) imaging OCT and angiography. To determine FAF, fluorescence was excited using diode laser at 488 nm. A fast retina map OCT was performed using the optic nerve as a landmark. IHC was performed to correlate with the findings of OCT and FAF changes.
Results: During the course of retinal degeneration, the FAF pattern evolved from some spotting at 2 months old to a mosaic of hyper fluorescent dots in rats 6 months and older. Retinal thicknesses progressively diminished over the course of the disease. At later stages of degeneration, OCT documented changes in the retinal layers, however, IHC better identified the cell loss and remodeling changes.
Angiography revealed attenuation of the retinal vascular plexus with time.
Conclusion: We provide for the first time a detailed long-term analysis of the course of retinal degeneration in P23H rats using a combination of SLO and OCT imaging, angiography, FAF and IHC. Although, the application of noninvasive methods enables longitudinal studies and will decrease thenumber of animals needed for a study, IHC is still an essential tool to identify retinal changes at the cellular level.
000070591 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B99$$9info:eu-repo/grantAgreement/ES/ISCIII/PI13-01124$$9info:eu-repo/grantAgreement/ES/ISCIII/RETICS-RD12-0034-0010$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2012-36845$$9info:eu-repo/grantAgreement/ES/UZ/UZ2011-BIO-02
000070591 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000070591 590__ $$a3.332$$b2016
000070591 591__ $$aOPHTHALMOLOGY$$b10 / 59 = 0.169$$c2016$$dQ1$$eT1
000070591 592__ $$a1.331$$b2016
000070591 593__ $$aOphthalmology$$c2016$$dQ1
000070591 593__ $$aSensory Systems$$c2016$$dQ2
000070591 593__ $$aCellular and Molecular Neuroscience$$c2016$$dQ3
000070591 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000070591 700__ $$aFernández Sánchez, Laura
000070591 700__ $$0(orcid)0000-0002-9250-9060$$aSegura, Francisco Javier$$uUniversidad de Zaragoza
000070591 700__ $$0(orcid)0000-0002-5621-1937$$aSánchez Cano, Ana Isabel$$uUniversidad de Zaragoza
000070591 700__ $$aTamarit, José María
000070591 700__ $$0(orcid)0000-0003-2656-6750$$aFuentes Broto, Lorena$$uUniversidad de Zaragoza
000070591 700__ $$aEells, Jt
000070591 700__ $$aLax, Pedro
000070591 700__ $$aCuenca, Nicolás
000070591 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología
000070591 7102_ $$11004$$2646$$aUniversidad de Zaragoza$$bDpto. Cirugía,Ginecol.Obstetr.$$cÁrea Oftalmología
000070591 7102_ $$12002$$2647$$aUniversidad de Zaragoza$$bDpto. Física Aplicada$$cÁrea Óptica
000070591 773__ $$g150 (2016), 122-134$$pExp. Eye Res.$$tExperimental Eye Research$$x0014-4835
000070591 8564_ $$s547538$$uhttps://zaguan.unizar.es/record/70591/files/texto_completo.pdf$$yPostprint
000070591 8564_ $$s58525$$uhttps://zaguan.unizar.es/record/70591/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
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000070591 951__ $$a2020-02-21-13:32:14
000070591 980__ $$aARTICLE