Self-sustained enzymatic cascade for the production of 2, 5-furandicarboxylic acid from 5-methoxymethylfurfural
Resumen: Background: 2, 5-Furandicarboxylic acid is a renewable building block for the production of polyfurandicarboxylates, which are biodegradable polyesters expected to substitute their classical counterparts derived from fossil resources. It may be produced from bio-based 5-hydroxymethylfurfural or 5-methoxymethylfurfural, both obtained by the acidic dehydration of biomass-derived fructose. 5-Methoxymethylfurfural, which is produced in the presence of methanol, generates less by-products and exhibits better storage stability than 5-hydroxymethylfurfural being, therefore, the industrial substrate of choice.
Results: In this work, an enzymatic cascade involving three fungal oxidoreductases has been developed for the production of 2, 5-furandicarboxylic acid from 5-methoxymethylfurfural. Aryl-alcohol oxidase and unspecific peroxygenase act on 5-methoxymethylfurfural and its partially oxidized derivatives yielding 2, 5-furandicarboxylic acid, as well as methanol as a by-product. Methanol oxidase takes advantage of the methanol released for in situ producing H2O2 that, along with that produced by aryl-alcohol oxidase, fuels the peroxygenase reactions. In this way, the enzymatic cascade proceeds independently, with the only input of atmospheric O2, to attain a 70% conversion of initial 5-methoxymethylfurfural. The addition of some exogenous methanol to the reaction further improves the yield to attain an almost complete conversion of 5-methoxymethylfurfural into 2, 5-furandicarboxylic acid.
Conclusions: The synergistic action of aryl-alcohol oxidase and unspecific peroxygenase in the presence of 5-methoxymethylfurfural and O2 is sufficient for the production of 2, 5-furandicarboxylic acid. The addition of methanol oxidase to the enzymatic cascade increases the 2, 5-furandicarboxylic acid yields by oxidizing a reaction by-product to fuel the peroxygenase reactions.

Idioma: Inglés
DOI: 10.1186/s13068-018-1091-2
Año: 2018
Publicado en: Biotechnology for Biofuels 11, 1 (2018), 86 [10 pp]
ISSN: 1754-6834

Financiación: info:eu-repo/grantAgreement/ES/MEC/FPU-AP2012-2041
Tipo y forma: Article (Published version)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
Exportado de SIDERAL (2019-03-26-11:48:10)

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 Notice créée le 2018-05-09, modifiée le 2019-03-26

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