000070331 001__ 70331
000070331 005__ 20200108100343.0
000070331 0247_ $$2doi$$a10.1186/s13068-018-1091-2
000070331 0248_ $$2sideral$$a105915
000070331 037__ $$aART-2018-105915
000070331 041__ $$aeng
000070331 100__ $$aCarro, J.
000070331 245__ $$aSelf-sustained enzymatic cascade for the production of 2, 5-furandicarboxylic acid from 5-methoxymethylfurfural
000070331 260__ $$c2018
000070331 5060_ $$aAccess copy available to the general public$$fUnrestricted
000070331 5203_ $$aBackground: 2, 5-Furandicarboxylic acid is a renewable building block for the production of polyfurandicarboxylates, which are biodegradable polyesters expected to substitute their classical counterparts derived from fossil resources. It may be produced from bio-based 5-hydroxymethylfurfural or 5-methoxymethylfurfural, both obtained by the acidic dehydration of biomass-derived fructose. 5-Methoxymethylfurfural, which is produced in the presence of methanol, generates less by-products and exhibits better storage stability than 5-hydroxymethylfurfural being, therefore, the industrial substrate of choice.
Results: In this work, an enzymatic cascade involving three fungal oxidoreductases has been developed for the production of 2, 5-furandicarboxylic acid from 5-methoxymethylfurfural. Aryl-alcohol oxidase and unspecific peroxygenase act on 5-methoxymethylfurfural and its partially oxidized derivatives yielding 2, 5-furandicarboxylic acid, as well as methanol as a by-product. Methanol oxidase takes advantage of the methanol released for in situ producing H2O2 that, along with that produced by aryl-alcohol oxidase, fuels the peroxygenase reactions. In this way, the enzymatic cascade proceeds independently, with the only input of atmospheric O2, to attain a 70% conversion of initial 5-methoxymethylfurfural. The addition of some exogenous methanol to the reaction further improves the yield to attain an almost complete conversion of 5-methoxymethylfurfural into 2, 5-furandicarboxylic acid.
Conclusions: The synergistic action of aryl-alcohol oxidase and unspecific peroxygenase in the presence of 5-methoxymethylfurfural and O2 is sufficient for the production of 2, 5-furandicarboxylic acid. The addition of methanol oxidase to the enzymatic cascade increases the 2, 5-furandicarboxylic acid yields by oxidizing a reaction by-product to fuel the peroxygenase reactions.
000070331 536__ $$9info:eu-repo/grantAgreement/ES/MEC/FPU-AP2012-2041
000070331 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000070331 590__ $$a5.452$$b2018
000070331 591__ $$aENERGY & FUELS$$b16 / 103 = 0.155$$c2018$$dQ1$$eT1
000070331 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b18 / 162 = 0.111$$c2018$$dQ1$$eT1
000070331 592__ $$a1.762$$b2018
000070331 593__ $$aApplied Microbiology and Biotechnology$$c2018$$dQ1
000070331 593__ $$aBiotechnology$$c2018$$dQ1
000070331 593__ $$aRenewable Energy, Sustainability and the Environment$$c2018$$dQ1
000070331 593__ $$aManagement, Monitoring, Policy and Law$$c2018$$dQ1
000070331 593__ $$aEnergy (miscellaneous)$$c2018$$dQ1
000070331 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000070331 700__ $$aFernández-Fueyo, E.
000070331 700__ $$aFernández-Alonso, C.
000070331 700__ $$aCañada, J.
000070331 700__ $$aUllrich, R.
000070331 700__ $$aHofrichter, M.
000070331 700__ $$aAlcalde, M.
000070331 700__ $$0(orcid)0000-0003-4076-6118$$aFerreira, P.$$uUniversidad de Zaragoza
000070331 700__ $$aMartínez, A.T.
000070331 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000070331 773__ $$g11, 1 (2018), 86 [10 pp]$$pBiotechnology for Biofuels$$tBiotechnology for Biofuels$$x1754-6834
000070331 8564_ $$s1575448$$uhttps://zaguan.unizar.es/record/70331/files/texto_completo.pdf$$yVersión publicada
000070331 8564_ $$s98284$$uhttps://zaguan.unizar.es/record/70331/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000070331 909CO $$ooai:zaguan.unizar.es:70331$$particulos$$pdriver
000070331 951__ $$a2020-01-08-09:28:50
000070331 980__ $$aARTICLE