Synthesis of a novel electrospun polycaprolactone scaffold functionalized with ibuprofen for periodontal regeneration: An in vitro and in vivo study
Resumen: Ibuprofen (IBU) has been shown to improve periodontal treatment outcomes. The aimof this study was to develop a new anti-inflammatory scaffold by functionalizing an electrospun nanofibrous poly-e-caprolactone membrane with IBU (IBU-PCL) and to evaluate its impact on periodontal inflammation, wound healing and regeneration in vitro and in vivo. IBU-PCL was synthesized through electrospinning. The effects of IBU-PCL on the proliferation and migration of epithelial cells (EC) and fibroblasts (FB) exposed to Porphyromonas gingivlais lipopolysaccharide (Pg-LPS) were evaluated through the AlamarBlue test and scratch assay, respectively. Anti-inflammatory and remodeling properties were investigated through Real time qPCR. Finally, the in vivo efficacy of the IBU-PCL membrane was assessed in an experimental periodontitis mouse model through histomorphometric analysis. The results showed that the anti-inflammatory effects of IBU on gingival cells were effectively amplified using the functionalizedmembrane. IBU-PCL reduced the proliferation and migration of cells challenged by Pg-LPS, as well as the expression of fibronectin-1, collagen-IV, integrin a3ß1 and laminin-5. In vivo, the membranes significantly improved the clinical attachment and IBU-PCL also reduced inflammation-induced bone destruction. These data showed that the IBU-PCL membrane could efficiently and differentially control inflammatory and migratory gingival cell responses and potentially promote periodontal regeneration.
Idioma: Inglés
DOI: 10.3390/ma11040580
Año: 2018
Publicado en: MATERIALS 11, 4 (2018), 580 [18 pp]
ISSN: 1996-1944

Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Ingeniería Química (Departamento de Ingeniería Química y Tecnologías del Medio Ambiente)

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Exportado de SIDERAL (2018-05-09-09:26:38)

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