000070281 001__ 70281
000070281 005__ 20200103141024.0
000070281 0247_ $$2doi$$a10.3390/ma11040580
000070281 0248_ $$2sideral$$a105903
000070281 037__ $$aART-2018-105903
000070281 041__ $$aeng
000070281 100__ $$aBatool, F.
000070281 245__ $$aSynthesis of a novel electrospun polycaprolactone scaffold functionalized with ibuprofen for periodontal regeneration: An in vitro and in vivo study
000070281 260__ $$c2018
000070281 5060_ $$aAccess copy available to the general public$$fUnrestricted
000070281 5203_ $$aIbuprofen (IBU) has been shown to improve periodontal treatment outcomes. The aimof this study was to develop a new anti-inflammatory scaffold by functionalizing an electrospun nanofibrous poly-e-caprolactone membrane with IBU (IBU-PCL) and to evaluate its impact on periodontal inflammation, wound healing and regeneration in vitro and in vivo. IBU-PCL was synthesized through electrospinning. The effects of IBU-PCL on the proliferation and migration of epithelial cells (EC) and fibroblasts (FB) exposed to Porphyromonas gingivlais lipopolysaccharide (Pg-LPS) were evaluated through the AlamarBlue test and scratch assay, respectively. Anti-inflammatory and remodeling properties were investigated through Real time qPCR. Finally, the in vivo efficacy of the IBU-PCL membrane was assessed in an experimental periodontitis mouse model through histomorphometric analysis. The results showed that the anti-inflammatory effects of IBU on gingival cells were effectively amplified using the functionalizedmembrane. IBU-PCL reduced the proliferation and migration of cells challenged by Pg-LPS, as well as the expression of fibronectin-1, collagen-IV, integrin a3ß1 and laminin-5. In vivo, the membranes significantly improved the clinical attachment and IBU-PCL also reduced inflammation-induced bone destruction. These data showed that the IBU-PCL membrane could efficiently and differentially control inflammatory and migratory gingival cell responses and potentially promote periodontal regeneration.
000070281 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000070281 590__ $$a2.972$$b2018
000070281 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b102 / 293 = 0.348$$c2018$$dQ2$$eT2
000070281 592__ $$a0.686$$b2018
000070281 593__ $$aMaterials Science (miscellaneous)$$c2018$$dQ2
000070281 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000070281 700__ $$aMorand, D.-N.
000070281 700__ $$aThomas, L.
000070281 700__ $$aBugueno, I.M.
000070281 700__ $$aAragon, J.$$uUniversidad de Zaragoza
000070281 700__ $$0(orcid)0000-0002-2966-9088$$aIrusta, S.$$uUniversidad de Zaragoza
000070281 700__ $$aKeller, L.
000070281 700__ $$aBenkirane-Jessel, N.
000070281 700__ $$aTenenbaum, H.
000070281 700__ $$aHuck, O.
000070281 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000070281 773__ $$g11, 4 (2018), 580 [18 pp]$$pMATERIALS$$tMaterials$$x1996-1944
000070281 8564_ $$s600887$$uhttps://zaguan.unizar.es/record/70281/files/texto_completo.pdf$$yVersión publicada
000070281 8564_ $$s97866$$uhttps://zaguan.unizar.es/record/70281/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000070281 909CO $$ooai:zaguan.unizar.es:70281$$particulos$$pdriver
000070281 951__ $$a2020-01-03-14:03:06
000070281 980__ $$aARTICLE