000070054 001__ 70054 000070054 005__ 20200117221639.0 000070054 0247_ $$2doi$$a10.1098/rsos.171592 000070054 0248_ $$2sideral$$a105456 000070054 037__ $$aART-2018-105456 000070054 041__ $$aeng 000070054 100__ $$aAlves, A.P. 000070054 245__ $$aGraph analysis of cell clusters forming vascular networks 000070054 260__ $$c2018 000070054 5060_ $$aAccess copy available to the general public$$fUnrestricted 000070054 5203_ $$aThis manuscript describes the experimental observation of vasculogenesis in chick embryos by means of network analysis. The formation of the vascular network was observed in the area opaca of embryos from 40 to 55 h of development. In the area opaca endothelial cell clusters self-organize as a primitive and approximately regular network of capillaries. The process was observed by bright-field microscopy in control embryos and in embryos treated with Bevacizumab (Avastin®), an antibody that inhibits the signalling of the vascular endothelial growth factor (VEGF). The sequence of images of the vascular growth were thresholded, and used to quantify the forming network in control and Avastin-treated embryos. This characterization is made by measuring vessels density, number of cell clusters and the largest cluster density. From the original images, the topology of the vascular network was extracted and characterized by means of the usual network metrics such as: the degree distribution, average clustering coefficient, average short path length and assortativity, among others. This analysis allows to monitor how the largest connected cluster of the vascular network evolves in time and provides with quantitative evidence of the disruptive effects that Avastin has on the tree structure of vascular networks. 000070054 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E19$$9info:eu-repo/grantAgreement/ES/MINECO/FIS2012-38266-C02-01$$9info:eu-repo/grantAgreement/ES/MINECO/FIS2014-55867-P 000070054 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000070054 590__ $$a2.515$$b2018 000070054 591__ $$aMULTIDISCIPLINARY SCIENCES$$b25 / 69 = 0.362$$c2018$$dQ2$$eT2 000070054 592__ $$a1.131$$b2018 000070054 593__ $$aMultidisciplinary$$c2018$$dQ1 000070054 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000070054 700__ $$aMesquita, O.N. 000070054 700__ $$0(orcid)0000-0002-3484-6413$$aGómez-Gardeñes, J.$$uUniversidad de Zaragoza 000070054 700__ $$aAgero, U. 000070054 7102_ $$12003$$2395$$aUniversidad de Zaragoza$$bDpto. Física Materia Condensa.$$cÁrea Física Materia Condensada 000070054 773__ $$g5, 3 (2018), 171592 [14 pp]$$pR. Soc. open sci.$$tRoyal Society Open Science$$x2054-5703 000070054 8564_ $$s1674382$$uhttps://zaguan.unizar.es/record/70054/files/texto_completo.pdf$$yVersión publicada 000070054 8564_ $$s70084$$uhttps://zaguan.unizar.es/record/70054/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000070054 909CO $$ooai:zaguan.unizar.es:70054$$particulos$$pdriver 000070054 951__ $$a2020-01-17-22:03:27 000070054 980__ $$aARTICLE