000069984 001__ 69984
000069984 005__ 20180419134225.0
000069984 0247_ $$2doi$$a10.1186/s12951-018-0354-1
000069984 0248_ $$2sideral$$a105429
000069984 037__ $$aART-2018-105429
000069984 041__ $$aeng
000069984 100__ $$aDalmau-Mena, I.
000069984 245__ $$aNanoparticles engineered to bind cellular motors for efficient delivery
000069984 260__ $$c2018
000069984 5060_ $$aAccess copy available to the general public$$fUnrestricted
000069984 5203_ $$aBackground: Dynein is a cytoskeletal molecular motor protein that transports cellular cargoes along microtubules. Biomimetic synthetic peptides designed to bind dynein have been shown to acquire dynamic properties such as cell accumulation and active intra- and inter-cellular motion through cell-to-cell contacts and projections to distant cells. On the basis of these properties dynein-binding peptides could be used to functionalize nanoparticles for drug delivery applications. Results: Here, we show that gold nanoparticles modified with dynein-binding delivery sequences become mobile, powered by molecular motor proteins. Modified nanoparticles showed dynamic properties, such as travelling the cytosol, crossing intracellular barriers and shuttling the nuclear membrane. Furthermore, nanoparticles were transported from one cell to another through cell-to-cell contacts and quickly spread to distant cells through cell projections. Conclusions: The capacity of these motor-bound nanoparticles to spread to many cells and increasing cellular retention, thus avoiding losses and allowing lower dosage, could make them candidate carriers for drug delivery.
000069984 536__ $$9info:eu-repo/grantAgreement/EUR/COST/CA15138 Transautophagy$$9info:eu-repo/grantAgreement/EUR/FP7/ERC-StG-239931-NANOPUZZLE$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2012-34533$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2015-69598-R$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-2-R
000069984 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000069984 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000069984 700__ $$0(orcid)0000-0002-1477-5259$$adel Pino, P.
000069984 700__ $$aPelaz, B.
000069984 700__ $$aCuesta-Geijo, M.
000069984 700__ $$aGalindo, I.
000069984 700__ $$0(orcid)0000-0002-2861-2469$$aMoros, M.
000069984 700__ $$0(orcid)0000-0003-1081-8482$$ade la Fuente, J.M.$$uUniversidad de Zaragoza
000069984 700__ $$aAlonso, C.
000069984 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDepartamento de Química Orgánica$$cQuímica Orgánica
000069984 773__ $$g16, 1 (2018), 33 [13 pp]$$pJ. nanobiotechnol.$$tJournal of nanobiotechnology$$x1477-3155
000069984 8564_ $$s2333888$$uhttp://zaguan.unizar.es/record/69984/files/texto_completo.pdf$$yVersión publicada
000069984 8564_ $$s101565$$uhttp://zaguan.unizar.es/record/69984/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000069984 909CO $$ooai:zaguan.unizar.es:69984$$particulos$$pdriver
000069984 951__ $$a2018-04-18-13:29:27
000069984 980__ $$aARTICLE