000069971 001__ 69971
000069971 005__ 20190709135543.0
000069971 0247_ $$2doi$$a10.1128/mBio.01961-16
000069971 0248_ $$2sideral$$a105151
000069971 037__ $$aART-2017-105151
000069971 041__ $$aeng
000069971 100__ $$aGhequire, M.G.K.
000069971 245__ $$aA Natural Chimeric Pseudomonas Bacteriocin with Novel Pore-Forming Activity Parasitizes the Ferrichrome Transporter
000069971 260__ $$c2017
000069971 5060_ $$aAccess copy available to the general public$$fUnrestricted
000069971 5203_ $$aModular bacteriocins represent a major group of secreted protein toxins with a narrow spectrum of activity, involved in interference competition between Gram-negative bacteria. These antibacterial proteins include a domain for binding to the target cell and a toxin module at the carboxy terminus. Self-inhibition of producers is provided by coexpression of linked immunity genes that transiently inhibit the toxin''s activity through formation of bacteriocin-immunity complexes or by insertion in the inner membrane, depending on the type of toxin module. We demonstrate strain-specific inhibitory activity for PmnH, a Pseudomonas bacteriocin with an unprecedented dual-toxin architecture, hosting both a colicin M domain, potentially interfering with peptidoglycan synthesis, and a novel colicin N-type domain, a pore-forming module distinct from the colicin Ia-type domain in Pseudomonas aeruginosa pyocin S5. A downstream-linked gene product confers PmnH immunity upon susceptible strains. This protein, ImnH, has a transmembrane topology similar to that of Pseudomonas colicin M-like and pore-forming immunity proteins, although homology with either of these is essentially absent. The enhanced killing activity of PmnH under iron-limited growth conditions reflects parasitism of the ferrichrome-type transporter for entry into target cells, a strategy shown here to be used as well by monodomain colicin M-like bacteriocins from pseudomonads. The integration of a second type of toxin module in a bacteriocin gene could offer a competitive advantage against bacteria displaying immunity against only one of both toxic activities.
000069971 536__ $$9info:eu-repo/grantAgreement/ES/MEC/Erasmus-800019
000069971 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000069971 590__ $$a6.689$$b2017
000069971 591__ $$aMICROBIOLOGY$$b13 / 125 = 0.104$$c2017$$dQ1$$eT1
000069971 592__ $$a4.106$$b2017
000069971 593__ $$aVirology$$c2017$$dQ1
000069971 593__ $$aMicrobiology$$c2017$$dQ1
000069971 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000069971 700__ $$aKemland, L.
000069971 700__ $$0(orcid)0000-0002-6649-9153$$aAnoz-Carbonell, E.$$uUniversidad de Zaragoza
000069971 700__ $$aBuchanan, S.K.
000069971 700__ $$aDe Mot, R.
000069971 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000069971 773__ $$g8, 1 (2017), e01961-16$$pMBIO$$tMBIO$$x2161-2129
000069971 8564_ $$s503931$$uhttps://zaguan.unizar.es/record/69971/files/texto_completo.pdf$$yVersión publicada
000069971 8564_ $$s97000$$uhttps://zaguan.unizar.es/record/69971/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000069971 909CO $$ooai:zaguan.unizar.es:69971$$particulos$$pdriver
000069971 951__ $$a2019-07-09-12:08:11
000069971 980__ $$aARTICLE