000069929 001__ 69929
000069929 005__ 20200716101419.0
000069929 0247_ $$2doi$$a10.1177/1464420717704880
000069929 0248_ $$2sideral$$a104429
000069929 037__ $$aART-2019-104429
000069929 041__ $$aeng
000069929 100__ $$0(orcid)0000-0003-2770-4617$$aOrtilles, A.
000069929 245__ $$aUse of 2% hydroxypropyl methylcellulose to prevent the corneal swelling during the in vitro mechanical characterization
000069929 260__ $$c2019
000069929 5060_ $$aAccess copy available to the general public$$fUnrestricted
000069929 5203_ $$aThe aim of this study was to assess the use of 2% HPMC during in vitro uniaxial tensile tests, with corneal strips immediately obtained or after storing the eyes for 24¿h in 0.9% NaCl solution at 4¿¿. The purpose was to establish a standardized procedure to prevent phenomena which can modify the mechanical properties of the tissue. Rabbit eyes were divided into four groups. Group A had seven eyes that were preserved in NaCl solution for 24¿h before testing. Group B had seven eyes that were immediately tested. In both groups, to prevent both swelling and dehydration, 2% hydroxypropyl methylcellulose (2% HPMC) was applied. Group C had seven eyes that were preserved in NaCl solution for 24¿h before testing. Group D had seven eyes that were immediately tested. In both groups, HPMC was not applied. Regarding the mechanical response, groups with HPMC showed similar Cauchy stress–stretch curves and there were no statistically significant differences at 5%, 10% and 15% strain between them, which mean that both showed similar mechanical behavior. The same result was obtained between groups without HPMC. However, for coupled groups with and without HPMC, statistically significant differences at 10% and 15% strain were observed. On the other hand, when grouped by storage time, statistically significant differences were found between groups that had eyes preserved for 24¿h with and without HPMC, respectively, as well as between groups immediately tested with and without HPMC, respectively, at 15% strain. Nevertheless, if coupled groups were considered, between groups that were preserved for 24¿h in NaCl before testing and groups that were immediately tested, no statistically significant differences were obtained. In addition, the Cauchy stress–stretch curves of groups without HPMC showed a decreasing slope of the linear part (strain¿>¿8%) of the graph during the experiment. In summary, the use of HPMC during the handling of the tissue from excision to testing seems to prevent both swelling and dehydration.
000069929 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/DPI2014-54981-R$$9info:eu-repo/grantAgreement/ES/MEC/FPU13-03782$$9info:eu-repo/grantAgreement/EC/FP7/606634/EU/Development of corneal biomechanical model. Dynamic topographical characterization based on 3D plenoptic imaging/POPCORN
000069929 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000069929 590__ $$a2.014$$b2019
000069929 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b193 / 314 = 0.615$$c2019$$dQ3$$eT2
000069929 592__ $$a0.376$$b2019
000069929 593__ $$aMechanical Engineering$$c2019$$dQ2
000069929 593__ $$aMaterials Science (miscellaneous)$$c2019$$dQ2
000069929 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000069929 700__ $$0(orcid)0000-0002-2959-0628$$aLanchares, E.$$uUniversidad de Zaragoza
000069929 700__ $$0(orcid)0000-0002-4295-8335$$aCristóbal, J.A.$$uUniversidad de Zaragoza
000069929 700__ $$0(orcid)0000-0001-9713-1813$$aCalvo, B.$$uUniversidad de Zaragoza
000069929 7102_ $$12007$$2265$$aUniversidad de Zaragoza$$bDpto. Métodos Estadísticos$$cÁrea Estadís. Investig. Opera.
000069929 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000069929 773__ $$g233, 5 (2019), 104429 [8 pp.]$$pProc. Inst. Mech. Eng., Part L, J. Mat.$$tPROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART L-JOURNAL OF MATERIALS-DESIGN AND APPLICATIONS$$x1464-4207
000069929 8564_ $$s588780$$uhttps://zaguan.unizar.es/record/69929/files/texto_completo.pdf$$yPostprint
000069929 8564_ $$s25854$$uhttps://zaguan.unizar.es/record/69929/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000069929 909CO $$ooai:zaguan.unizar.es:69929$$particulos$$pdriver
000069929 951__ $$a2020-07-16-08:38:56
000069929 980__ $$aARTICLE