000069429 001__ 69429
000069429 005__ 20181017144516.0
000069429 0247_ $$2doi$$a10.1128/AEM.00469-13
000069429 0248_ $$2sideral$$a82652
000069429 037__ $$aART-2013-82652
000069429 041__ $$aeng
000069429 100__ $$0(orcid)0000-0002-5895-2157$$aGayán, E.$$uUniversidad de Zaragoza
000069429 245__ $$aEffect of pressure-induced changes in the ionization equilibria of buffers on inactivation of Escherichia coli and staphylococcus aureus by high hydrostatic pressure
000069429 260__ $$c2013
000069429 5060_ $$aAccess copy available to the general public$$fUnrestricted
000069429 5203_ $$aSurvival rates of Escherichia coli and Staphylococcus aureus after high-pressure treatment in buffers that had large or small reaction volumes (¿V°), and which therefore underwent large or small changes in pH under pressure, were compared. At a low buffer concentration of 0.005 M, survival was, as expected, better in MOPS (morpholinepropanesulfonic acid), HEPES, and Tris, whose ¿V° values are approximately 5.0 to 7.0 cm3 mol-1, than in phosphate or dimethyl glutarate (DMG), whose ¿V° values are about -25 cm3 mol-1. However, at a concentration of 0.1 M, survival was unexpectedly better in phosphate and DMG than in MOPS, HEPES, or Tris. This was because the baroprotective effect of phosphate and DMG increased much more rapidly with increasing concentration than it did with MOPS, HEPES, or Tris. Further comparisons of survival in solutions of salts expected to cause large electrostriction effects (Na2SO4 and CaCl2) and those causing lower electrostriction (NaCl and KCl) were made. The salts with divalent ions were protective at much lower concentrations than salts with monovalent ions. Buffers and salts both protected against transient membrane disruption in E. coli, but the molar concentrations necessary for membrane protection were much lower for phosphate and Na2SO4 than for HEPES and NaCl. Possible protective mechanisms discussed include effects of electrolytes on water compressibility and kosmotropic and specific ion effects. The results of this systematic study will be of considerable practical significance in studies of pressure inactivation of microbes under defined conditions but also raise important fundamental questions regarding the mechanisms of baroprotection by ionic solutes.
000069429 536__ $$9info:eu-repo/grantAgreement/ES/CICYT-FEDER/CIT-020000-2009-40
000069429 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000069429 590__ $$a3.952$$b2013
000069429 591__ $$aMICROBIOLOGY$$b24 / 119 = 0.202$$c2013$$dQ1$$eT1
000069429 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b30 / 165 = 0.182$$c2013$$dQ1$$eT1
000069429 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000069429 700__ $$0(orcid)0000-0002-1902-0648$$aCondón, S.$$uUniversidad de Zaragoza
000069429 700__ $$0(orcid)0000-0003-2430-858X$$aÁlvarez, I.$$uUniversidad de Zaragoza
000069429 700__ $$aNabakabaya, M.
000069429 700__ $$aMackey, B.
000069429 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000069429 773__ $$g79, 13 (2013), 4041-4047$$pAppl. environ. microbiol.$$tAPPLIED AND ENVIRONMENTAL MICROBIOLOGY$$x0099-2240
000069429 8564_ $$s154919$$uhttps://zaguan.unizar.es/record/69429/files/texto_completo.pdf$$yVersión publicada
000069429 8564_ $$s130106$$uhttps://zaguan.unizar.es/record/69429/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000069429 909CO $$ooai:zaguan.unizar.es:69429$$particulos$$pdriver
000069429 951__ $$a2018-10-17-14:44:03
000069429 980__ $$aARTICLE