000065620 001__ 65620
000065620 005__ 20190709135447.0
000065620 0247_ $$2doi$$a10.1016/j.actbio.2017.01.001
000065620 0248_ $$2sideral$$a98164
000065620 037__ $$aART-2017-98164
000065620 041__ $$aeng
000065620 100__ $$aKuchler-Bopp, S.
000065620 245__ $$aPromoting bioengineered tooth innervation using nanostructured and hybrid scaffolds
000065620 260__ $$c2017
000065620 5060_ $$aAccess copy available to the general public$$fUnrestricted
000065620 5203_ $$aThe innervation of teeth mediated by axons originating from the trigeminal ganglia is essential for their function and protection. Immunosuppressive therapy using Cyclosporine A (CsA) was found to accelerate the innervation of transplanted tissues and particularly that of bioengineered teeth. To avoid the CsA side effects, we report in this study the preparation of CsA loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles, their embedding on polycaprolactone (PCL)-based scaffolds and their possible use as templates for the innervation of bioengineered teeth. This PCL scaffold, approved by the FDA and capable of mimicking the extracellular matrix, was obtained by electrospinning and decorated with CsA-loaded PLGA nanoparticles to allow a local sustained action of this immunosuppressive drug. Dental re-associations were co-implanted with a trigeminal ganglion on functionalized scaffolds containing PLGA and PLGA/cyclosporine in adult ICR mice during 2 weeks. Histological analyses showed that the designed scaffolds did not alter the teeth development after in vivo implantation. The study of the innervation of the dental re-associations by indirect immunofluorescence and transmission electron microscopy (TEM), showed that 88.4% of the regenerated teeth were innervated when using the CsA-loaded PLGA scaffold. The development of active implants thus allows their potential use in the context of dental engineering. Statement of Significance Tooth innervation is essential for their function and protection and this can be promoted in vivo using polymeric scaffolds functionalized with immunosuppressive drug-loaded nanoparticles. Immunosuppressive therapy using biodegradable nanoparticles loaded with Cyclosporine A was found to accelerate the innervation of bioengineered teeth after two weeks of implantation.
000065620 536__ $$9info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM
000065620 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000065620 590__ $$a6.383$$b2017
000065620 591__ $$aMATERIALS SCIENCE, BIOMATERIALS$$b3 / 33 = 0.091$$c2017$$dQ1$$eT1
000065620 591__ $$aENGINEERING, BIOMEDICAL$$b4 / 78 = 0.051$$c2017$$dQ1$$eT1
000065620 592__ $$a1.967$$b2017
000065620 593__ $$aBiochemistry$$c2017$$dQ1
000065620 593__ $$aBiomaterials$$c2017$$dQ1
000065620 593__ $$aBiomedical Engineering$$c2017$$dQ1
000065620 593__ $$aBiotechnology$$c2017$$dQ1
000065620 593__ $$aMedicine (miscellaneous)$$c2017$$dQ1
000065620 593__ $$aMolecular Biology$$c2017$$dQ2
000065620 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000065620 700__ $$0(orcid)0000-0002-6277-7996$$aLarrea, A.
000065620 700__ $$aPetry, L.
000065620 700__ $$aIdoux-Gillet, Y.
000065620 700__ $$0(orcid)0000-0002-6873-5244$$aSebastian, V.$$uUniversidad de Zaragoza
000065620 700__ $$aFerrandon, A.
000065620 700__ $$aSchwinté, P.
000065620 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, M.$$uUniversidad de Zaragoza
000065620 700__ $$aBenkirane-Jessel, N.
000065620 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000065620 773__ $$g50 (2017), 493-501$$pActa Biomater.$$tACTA BIOMATERIALIA$$x1742-7061
000065620 8564_ $$s1344542$$uhttps://zaguan.unizar.es/record/65620/files/texto_completo.pdf$$yPostprint
000065620 8564_ $$s55467$$uhttps://zaguan.unizar.es/record/65620/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000065620 909CO $$ooai:zaguan.unizar.es:65620$$particulos$$pdriver
000065620 951__ $$a2019-07-09-11:38:41
000065620 980__ $$aARTICLE