000065249 001__ 65249
000065249 005__ 20180222100135.0
000065249 0247_ $$2doi$$a10.3390/fib5030034
000065249 0248_ $$2sideral$$a104348
000065249 037__ $$aART-2017-104348
000065249 041__ $$aeng
000065249 100__ $$aMaione, S.
000065249 245__ $$aAntimicrobial electrospun fibers of polyester loaded with engineered cyclic gramicidin analogues
000065249 260__ $$c2017
000065249 5060_ $$aAccess copy available to the general public$$fUnrestricted
000065249 5203_ $$aBiodegradable polyester fibers have been loaded with two engineered analogues of gramicidin soviet. In these cyclic peptide derivatives, which were designed in a previous work to stabilize the bioactive conformation while enhancing the antimicrobial activity, the D-Phe was replaced by D-Pro, and the L-Pro was changed by 1-aminocyclopropanecarboxylic acid (Ac3c) or by an Ac3c derivative with two vicinal phenyl substituents in a trans relative disposition (S, S-c3diPhe). The diameter, topography, thermal stability and wettability of the polyester fibers, which have been obtained by electrospinning, strongly depend on the molecular constraints and stability of the loaded peptides. More specifically, unloaded and linear gramicidin-loaded fibers (used as control) are hydrophobic, rough and micrometric, while fibers loaded with the cyclic peptides are hydrophilic, ultra-smooth, nanometric and less thermally stable. The activity of the two cyclic peptides increases when loaded into polyester fibers, suggesting that the polymeric matrix stabilizes the bioactive ß-sheet structure. The peptide with S, S-c3diPhe displays higher antibiotic potency and biocompatibility than that with Ac3c, which indicates not only that the bioactive conformation is better preserved by the former but also the significant role played by the phenyl rings in the recognition by living cells.
000065249 536__ $$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/MAT2015-69547-R$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/MAT2015-69367-R$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/CTQ2013-40855-R$$9info:eu-repo/grantAgreement/ES/DGA/E40
000065249 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000065249 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000065249 700__ $$adel Valle, L.J.
000065249 700__ $$aPérez-Madrigal, M.M.
000065249 700__ $$0(orcid)0000-0003-3222-0828$$aCativiela, C.$$uUniversidad de Zaragoza
000065249 700__ $$aPuiggalí, J.
000065249 700__ $$aAlemán, C.
000065249 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDepartamento de Química Orgánica$$cQuímica Orgánica
000065249 773__ $$g5, 3 (2017), 34 [20 pp]$$tFibers$$x2079-6439
000065249 8564_ $$s1380640$$uhttp://zaguan.unizar.es/record/65249/files/texto_completo.pdf$$yVersión publicada
000065249 8564_ $$s105510$$uhttp://zaguan.unizar.es/record/65249/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000065249 909CO $$ooai:zaguan.unizar.es:65249$$particulos$$pdriver
000065249 951__ $$a2018-02-22-09:58:50
000065249 980__ $$aARTICLE