000064422 001__ 64422
000064422 005__ 20180118124054.0
000064422 0247_ $$2doi$$a10.17265/2161-6256/2016.05.008
000064422 0248_ $$2sideral$$a103722
000064422 037__ $$aART-2016-103722
000064422 041__ $$aeng
000064422 100__ $$ade Brun, V.
000064422 245__ $$aHepatic Expression of Insulin-Like Growth Factor-1 in Underfed Pregnant Ewes
000064422 260__ $$c2016
000064422 5060_ $$aAccess copy available to the general public$$fUnrestricted
000064422 5203_ $$aThe liver is one of the most important visceral organs, which represents a large contribution to whole animal energy expenditure and the major synthetic site of insulin-like growth factor-1 (IGF-1) peptide. Decreased plane of nutrition acts by reducing the metabolic rate and mass of metabolic tissues, such as liver. Also, undernutrition results in the reduced circulating IGF-1 concentrations, due to the uncoupled growth hormone-IGF (GH-IGF) axis. This study investigated whether a 22-day period of undernutrition (half maintenance) could affect liver mass and IGF-1 protein and gene expression. Sixteen pregnant ewes fed all (n = 9) or half (n = 7) of their maintenance energy requirements were slaughtered on day 7 of pregnancy (oestrus = day 0). Body and liver mass, IGF-1 plasmatic concentrations and liver IGF-1 mRNA and protein expression were determined. Liver mass and the proportion of liver mass to empty body weight were lower in underfed animals. While IGF-1 plasmatic concentrations were lower in undernourished ewes, no differences in liver mRNA expression were found. This is the first time that differences in immunohistochemistry intensity and total content are reported in sheep. In summary, the decreased plasma IGF-1 concentrations induced by undernutrition in ewes was not associated with its reduced hepatic mRNA or protein expression, but to a decrease in liver mass.
000064422 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A26$$9info:eu-repo/grantAgreement/ES/MICINN/AGL2010-15004-GAN
000064422 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000064422 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000064422 700__ $$aMeikle, A.
000064422 700__ $$aCasal, A.
000064422 700__ $$aCarriquiry, M.
000064422 700__ $$aMenezes, C.
000064422 700__ $$0(orcid)0000-0002-5399-8746$$aForcada, F.$$uUniversidad de Zaragoza
000064422 700__ $$0(orcid)0000-0003-2907-0427$$aSosa, C$$uUniversidad de Zaragoza
000064422 700__ $$0(orcid)0000-0003-2827-3054$$aAbecia, J.A.$$uUniversidad de Zaragoza
000064422 7102_ $$11000$$2807$$aUniversidad de Zaragoza$$bDepartamento de Anatomía Patológica, Medicina Legal y Forense y Toxicología$$cToxicología
000064422 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDepartamento de Producción Animal y Ciencia de los Alimentos$$cProducción Animal
000064422 773__ $$g6 (2016), 355-362$$pJ. agr. sci.technol. A$$tJournal of Agricultural Science and Technology A$$x2161-6256
000064422 8564_ $$s198766$$uhttp://zaguan.unizar.es/record/64422/files/texto_completo.pdf$$yVersión publicada
000064422 8564_ $$s100036$$uhttp://zaguan.unizar.es/record/64422/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000064422 909CO $$ooai:zaguan.unizar.es:64422$$particulos$$pdriver
000064422 951__ $$a2018-01-17-14:08:33
000064422 980__ $$aARTICLE