000063514 001__ 63514
000063514 005__ 20190709135626.0
000063514 0247_ $$2doi$$a10.14670/HH-sehit17
000063514 0248_ $$2sideral$$a103263
000063514 037__ $$aART-2017-103263
000063514 041__ $$aeng
000063514 100__ $$0(orcid)0000-0002-4907-8744$$aGracia LLanes, Javier$$uUniversidad de Zaragoza
000063514 245__ $$aUltrastructural characteristics of Glial and Interstitial cells of Cajal in the Lizard intestine: presence of primary cilium
000063514 260__ $$c2017
000063514 5060_ $$aAccess copy available to the general public$$fUnrestricted
000063514 5203_ $$aIn mammals, enteric nervous system consists of neurons and glial cells which are mainly organized in two ganglionated plexuses: the myenteric plexus, and the inner and outer submucosal plexuses. Enteric glial cells establish a direct contact with neurons; their cytoplasm prolongations surround the unmyelinated axons. The interstitial cells of Cajal (ICCs) are located around enteric ganglia and in both muscular layers forming an interconnected network. It is now known that some ICCs and glial cells in mammals present a single cilium but its presence in the rest of vertebrate classes has been not still demonstrated. The aim of the present study is to characterize, at the ultrastructural level, the enteric glial cells and ICCs in the lizard intestine.
Material and methods: We used ten adult lizards Podareis hispanica (Reptilia). Small and large intestine wall samples were fixed with 2.5% glutaraldehyde in PB buffer (pH 7.3) and routinely processed for TEM visualization.
Results: Lizard intestinal ganglia show a lower number of neurons than those from mammals. Glial cells were smaller than neurons and they showed dark nuclei because of their condénsate heterochromatin. These glial cells surround axons mainly containing mixed both cholinergic and peptidergic vesicles. Occasionally, some axons were found surrounded by myelin sheaths. We found ICCs around enteric ganglia of the myenteric plexus. They present triangular or spindle forms and a very voluminous nucleus, with scarce marginal heterochromatin, surrounded by a thin perinuclear cytoplasm that expands with long cytoplasmic processes. ICC processes penetrate and connect with other ICCs located in the connective tissue of the both muscle layers by gap-like junctions forming a three-dimensional network. In addition, we demónstrate the presence of a primary cilium in ICCs as well as in glial cells. We describe their ultraestructural features: basal foot and cap in the basal corpuscle, (9+0) axonema, and a characteristic domain of membrane: the ciliary pocket.
Conclusions: Our data support that the single cilium is present in both kinds of cells and consequently, this is a phylogenetically preserved structure.
000063514 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000063514 590__ $$a2.015$$b2017
000063514 591__ $$aPATHOLOGY$$b40 / 79 = 0.506$$c2017$$dQ3$$eT2
000063514 591__ $$aCELL BIOLOGY$$b151 / 190 = 0.795$$c2017$$dQ4$$eT3
000063514 592__ $$a0.672$$b2017
000063514 593__ $$aPathology and Forensic Medicine$$c2017$$dQ2
000063514 593__ $$aHistology$$c2017$$dQ2
000063514 655_4 $$ainfo:eu-repo/semantics/conferenceObject$$vinfo:eu-repo/semantics/publishedVersion
000063514 700__ $$0(orcid)0000-0002-2787-9671$$aMonzón, Marta$$uUniversidad de Zaragoza
000063514 700__ $$aIruzubieta, Pablo
000063514 700__ $$aBerga, Carmen
000063514 700__ $$0(orcid)0000-0003-4071-1467$$aLuesma, Mª José$$uUniversidad de Zaragoza
000063514 700__ $$0(orcid)0000-0002-9951-1075$$aJunquera, Mª Concepción$$uUniversidad de Zaragoza
000063514 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000063514 7102_ $$11003$$2027$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Anatom.Embriol.Humana
000063514 773__ $$g32 , Suppl. 1 (2017), 66$$pHistol. histopathol.$$tHistology and histopathology$$x0213-3911
000063514 8564_ $$s721234$$uhttps://zaguan.unizar.es/record/63514/files/texto_completo.pdf$$yVersión publicada
000063514 8564_ $$s91994$$uhttps://zaguan.unizar.es/record/63514/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000063514 909CO $$ooai:zaguan.unizar.es:63514$$particulos$$pdriver
000063514 951__ $$a2019-07-09-12:31:03
000063514 980__ $$aARTICLE