000063274 001__ 63274
000063274 005__ 20240122154813.0
000063274 0247_ $$2doi$$a10.1371/journal.pone.0055231
000063274 0248_ $$2sideral$$a90650
000063274 037__ $$aART-2013-90650
000063274 041__ $$aeng
000063274 100__ $$0(orcid)0000-0002-8100-5596$$aMartínez-Beamonte, R$$uUniversidad de Zaragoza
000063274 245__ $$aPostprandial changes in high density lipoproteins in rats Subjected to Gavage Administration of Virgin Olive Oil
000063274 260__ $$c2013
000063274 5060_ $$aAccess copy available to the general public$$fUnrestricted
000063274 5203_ $$aBackground and Aims: The present study was designed to verify the influence of acute fat loading on high density lipoprotein (HDL) composition, and the involvement of liver and different segments of small intestine in the changes observed. Methods and Results: To address these issues, rats were administered a bolus of 5-ml of extra-virgin olive oil and sacrificed 4 and 8 hours after feeding. In these animals, lipoproteins were analyzed and gene expressions of apolipoprotein and HDL enzymes were assessed in duodenum, jejunum, ileum and liver. Using this experimental design, total plasma and HDL phospholipids increased at the 8-hour-time-point due to increased sphingomyelin content. An increase in apolipoprotein A4 was also observed mainly in lipid-poor HDL. Increased expression of intestinal Apoa1, Apoa4 and Sgms1 mRNA was accompanied by hepatic decreases in the first two genes in liver. Hepatic expression of Abcg1, Apoa1bp, Apoa2, Apoe, Ptlp, Pon1 and Scarb1 decreased significantly following fat gavage, while no changes were observed for Abca1, Lcat or Pla2g7. Significant associations were also noted for hepatic expression of apolipoproteins and Pon1. Manipulation of postprandial triglycerides using an inhibitor of microsomal transfer protein -CP-346086- or of lipoprotein lipase –tyloxapol- did not influence hepatic expression of Apoa1 or Apoa4 mRNA. Conclusion: All these data indicate that dietary fat modifies the phospholipid composition of rat HDL, suggesting a mechanism of down-regulation of hepatic HDL when intestine is the main source of those particles and a coordinated regulation of hepatic components of these lipoproteins at the mRNA level, independently of plasma postprandial triglycerides.
000063274 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B69$$9info:eu-repo/grantAgreement/ES/DGA/PI025-08$$9info:eu-repo/grantAgreement/ES/MICINN/SAF2010-14958
000063274 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000063274 590__ $$a3.534$$b2013
000063274 591__ $$aMULTIDISCIPLINARY SCIENCES$$b8 / 56 = 0.143$$c2013$$dQ1$$eT1
000063274 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000063274 700__ $$0(orcid)0000-0002-0108-1004$$aNavarro, Maria.A$$uUniversidad de Zaragoza
000063274 700__ $$aAcín, S
000063274 700__ $$0(orcid)0000-0001-6627-298X$$aGuillén, N$$uUniversidad de Zaragoza
000063274 700__ $$0(orcid)0000-0002-8442-8041$$aBarranquero, C
000063274 700__ $$0(orcid)0000-0003-1197-4276$$aArnal, C$$uUniversidad de Zaragoza
000063274 700__ $$0(orcid)0000-0002-5841-0462$$aSurra, J$$uUniversidad de Zaragoza
000063274 700__ $$0(orcid)0000-0002-8251-8457$$aOsada, J.$$uUniversidad de Zaragoza
000063274 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000063274 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000063274 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000063274 7102_ $$11000$$2807$$aUniversidad de Zaragoza$$bDpto. Anat.Pat.Med.Leg.For.To.$$cArea Toxicología
000063274 773__ $$g8, 1 (2013), e55231 [10 pp]$$pPLoS One$$tPLoS ONE$$x1932-6203
000063274 8564_ $$s684905$$uhttps://zaguan.unizar.es/record/63274/files/texto_completo.pdf$$yVersión publicada
000063274 8564_ $$s122550$$uhttps://zaguan.unizar.es/record/63274/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000063274 909CO $$ooai:zaguan.unizar.es:63274$$particulos$$pdriver
000063274 951__ $$a2024-01-22-15:30:36
000063274 980__ $$aARTICLE