000063228 001__ 63228
000063228 005__ 20240104111806.0
000063228 0247_ $$2doi$$a10.4142/jvs.2017.18.1.39
000063228 0248_ $$2sideral$$a98437
000063228 037__ $$aART-2017-98437
000063228 041__ $$aeng
000063228 100__ $$0(orcid)0000-0001-9818-508X$$aBarrachina, L.$$uUniversidad de Zaragoza
000063228 245__ $$aInflammation affects the viability and plasticity of equine mesenchymal stem cells: Possible implications in intra-articular treatments
000063228 260__ $$c2017
000063228 5060_ $$aAccess copy available to the general public$$fUnrestricted
000063228 5203_ $$aMesenchymal stem cells (MSCs) are gaining relevance for treating equine joint injuries because of their ability to limit inflammation and stimulate regeneration. Because inflammation activates MSC immunoregulatory function, proinflammatory priming could improve MSC efficacy. However, inflammatory molecules present in synovial fluid or added to the culture medium might have deleterious effects on MSCs. Therefore, this study was conducted to investigate the effects of inflammatory synovial fluid and proinflammatory cytokines priming on viability and plasticity of equine MSCs. Equine bone marrow derived MSCs (eBM-MSCs) from three animals were cultured for 72 h in media supplemented with: 20% inflammatory synovial fluid (SF); 50 ng/mL IFN-¿ and TNF-a (CK50); and 20 ng/mL IFN-¿ and TNF-a (CK20). Proliferation assay and expression of proliferation and apoptosis-related genes showed that SF exposed-eBM-MSCs maintained their viability, whereas the viability of CK primed-eBM-MSCs was significantly impaired. Tri-lineage differentiation assay revealed that exposure to inflammatory synovial fluid did not alter eBM-MSCs differentiation potential; however, eBM-MSCs primed with cytokines did not display osteogenic, adipogenic or chondrogenic phenotype. The inflammatory synovial environment is well tolerated by eBM-MSCs, whereas cytokine priming negatively affects the viability and differentiation abilities of eBM-MSCs, which might limit their in vivo efficacy.
000063228 536__ $$9info:eu-repo/grantAgreement/ES/DGA/LAGENBIO-GROUP$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2011-28609
000063228 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-sa$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/es/
000063228 590__ $$a1.327$$b2017
000063228 591__ $$aVETERINARY SCIENCES$$b48 / 139 = 0.345$$c2017$$dQ2$$eT2
000063228 592__ $$a0.469$$b2017
000063228 593__ $$aVeterinary (miscellaneous)$$c2017$$dQ2
000063228 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000063228 700__ $$0(orcid)0000-0002-1075-8267$$aRemacha, A. R.$$uUniversidad de Zaragoza
000063228 700__ $$0(orcid)0000-0001-7188-0461$$aRomero, A.$$uUniversidad de Zaragoza
000063228 700__ $$0(orcid)0000-0002-8712-2275$$aVázquez, F. J.$$uUniversidad de Zaragoza
000063228 700__ $$0(orcid)0000-0003-4489-3130$$aAlbareda, J.$$uUniversidad de Zaragoza
000063228 700__ $$0(orcid)0000-0003-2713-9232$$aPrades, M.
000063228 700__ $$0(orcid)0000-0002-4495-8857$$aRanera, B.
000063228 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, P.$$uUniversidad de Zaragoza
000063228 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, I.$$uUniversidad de Zaragoza
000063228 700__ $$0(orcid)0000-0003-3289-2675$$aRodellar, C.$$uUniversidad de Zaragoza
000063228 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000063228 7102_ $$11004$$2830$$aUniversidad de Zaragoza$$bDpto. Cirugía,Ginecol.Obstetr.$$cÁrea Traumatología y Ortopedia
000063228 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000063228 773__ $$g18, 1 (2017), 39-49$$pJournal of Veterinary Science$$tJournal of Veterinary Science$$x1229-845X
000063228 8564_ $$s6609971$$uhttps://zaguan.unizar.es/record/63228/files/texto_completo.pdf$$yVersión publicada
000063228 8564_ $$s111331$$uhttps://zaguan.unizar.es/record/63228/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000063228 909CO $$ooai:zaguan.unizar.es:63228$$particulos$$pdriver
000063228 951__ $$a2024-01-04-11:00:31
000063228 980__ $$aARTICLE