000063224 001__ 63224
000063224 005__ 20200428120341.0
000063224 0247_ $$2doi$$a10.2527/jas.2016.1286
000063224 0248_ $$2sideral$$a98852
000063224 037__ $$aART-2017-98852
000063224 041__ $$aeng
000063224 100__ $$aBarranco, I.
000063224 245__ $$aProfile and reproductive roles of seminal plasma melatonin of boar ejaculates used in artificial insemination programs
000063224 260__ $$c2017
000063224 5060_ $$aAccess copy available to the general public$$fUnrestricted
000063224 5203_ $$aMelatonin (MLT) is present in seminal plasma (SP) of mammalian species, including pigs, and it is credited with antioxidant properties. This study aims to identify the sources of variation and the role of boar SP MLT on sperm quality and functionality and in vivo fertilizing ability of liquid-stored semen doses used in AI programs. The SP MLT was measured using an ELISA kit in a total of 219 ejaculates collected from 76 boars, and reproductive records of 5,318 AI sows were recorded. Sperm quality was assessed according to motility (computer-aided sperm analysis) and viability (cytometry evaluation). Sperm functionality was assessed according to the cytometric determination of intracellular H2O2 generation, total and mitochondrial O2- production, and lipid peroxidation in liquid AI semen samples stored at 17°C over 144 h. The concentration of SP MLT differed among seasons (P < 0.01) and day length periods (P < 0.001) of the year, demonstrating that the ejaculates collected during the increasing day length period (9.80 ± 1.38 pg/mL, range: 2.75–21.94) had lower SP MLT concentrations than those collected during the decreasing day length period (16.32 ± 1.67 pg/mL, range: 5.02–35.61). The SP MLT also differed (P < 0.001) among boars, among ejaculates within boar, and among portions within the ejaculate, demonstrating that SP from the first 10 mL of sperm-rich ejaculate fraction (SRF) exhibited lower MLT concentrations than post-SRF. The SP MLT was negatively related (P < 0.001) to mitochondrial O2- production in viable sperm. The SP MLT did not differ among AI boars (n = 14) hierarchically grouped according to high and low fertility outcomes. In conclusion, SP MLT concentration in AI boars varies depending on the season of ejaculate collection and differs among boars, ejaculates within boar, and portions within ejaculate. The SP MLT may act at the mitochondrial level of sperm by reducing the generation of O2-. However, this antioxidant role of SP MLT was not reflected in sperm quality or in vivo fertility outcomes of AI semen doses.
000063224 536__ $$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL2013-43328-P$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2015-69738-R$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2012-39903
000063224 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000063224 590__ $$a1.711$$b2017
000063224 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b12 / 59 = 0.203$$c2017$$dQ1$$eT1
000063224 592__ $$a0.848$$b2017
000063224 593__ $$aAnimal Science and Zoology$$c2017$$dQ1
000063224 593__ $$aFood Science$$c2017$$dQ1
000063224 593__ $$aMedicine (miscellaneous)$$c2017$$dQ2
000063224 593__ $$aGenetics$$c2017$$dQ3
000063224 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000063224 700__ $$0(orcid)0000-0003-1997-4262$$aCasao, A.$$uUniversidad de Zaragoza
000063224 700__ $$aPerez-Patiño, C.
000063224 700__ $$aParrilla, I.
000063224 700__ $$0(orcid)0000-0003-1306-8517$$aMuiño-Blanco, T.$$uUniversidad de Zaragoza
000063224 700__ $$aMartinez, E.A.
000063224 700__ $$0(orcid)0000-0001-9842-6197$$aCebrian-Perez, J.A.$$uUniversidad de Zaragoza
000063224 700__ $$aRoca, J.
000063224 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000063224 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000063224 773__ $$g95, 4 (2017), 1660-1668$$pJ. anim. sci.$$tJOURNAL OF ANIMAL SCIENCE$$x0021-8812
000063224 8564_ $$s452570$$uhttps://zaguan.unizar.es/record/63224/files/texto_completo.pdf$$yVersión publicada
000063224 8564_ $$s114987$$uhttps://zaguan.unizar.es/record/63224/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000063224 909CO $$ooai:zaguan.unizar.es:63224$$particulos$$pdriver
000063224 951__ $$a2020-04-28-12:01:18
000063224 980__ $$aARTICLE