000063145 001__ 63145
000063145 005__ 20200324135213.0
000063145 0247_ $$2doi$$a10.1038/s41598-017-12049-4
000063145 0248_ $$2sideral$$a101469
000063145 037__ $$aART-2017-101469
000063145 041__ $$aeng
000063145 100__ $$0(orcid)0000-0003-3660-8651$$aVirumbrales-Muñoz, Maria$$uUniversidad de Zaragoza
000063145 245__ $$aMultiwell capillarity-based microfluidic device for the study of 3D tumour tissue-2D endothelium interactions and drug screening in co-culture models
000063145 260__ $$c2017
000063145 5060_ $$aAccess copy available to the general public$$fUnrestricted
000063145 5203_ $$aThe tumour microenvironment is very complex, and essential in tumour development and drug resistance. The endothelium is critical in the tumour microenvironment: it provides nutrients and oxygen to the tumour and is essential for systemic drug delivery. Therefore, we report a simple, user-friendly microfluidic device for co-culture of a 3D breast tumour model and a 2D endothelium model for cross-talk and drug delivery studies. First, we demonstrated the endothelium was functional, whereas the tumour model exhibited in vivo features, e.g., oxygen gradients and preferential proliferation of cells with better access to nutrients and oxygen. Next, we observed the endothelium structure lost its integrity in the co-culture. Following this, we evaluated two drug formulations of TRAIL (TNF-related apoptosis inducing ligand): soluble and anchored to a LUV (large unilamellar vesicle). Both diffused through the endothelium, LUV-TRAIL being more efficient in killing tumour cells, showing no effect on the integrity of endothelium. Overall, we have developed a simple capillary force-based microfluidic device for 2D and 3D cell co-cultures. Our device allows high-throughput approaches, patterning different cell types and generating gradients without specialised equipment. We anticipate this microfluidic device will facilitate drug screening in a relevant microenvironment thanks to its simple, effective and user-friendly operation.
000063145 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/MTM2012-31073$$9info:eu-repo/grantAgreement/ES/MINECO/DPI2011-28262-C04-01$$9info:eu-repo/grantAgreement/ES/MINECO/DPI2015-65401-C3-1-R$$9info:eu-repo/grantAgreement/ES/MINECO/MAT2015-69967-C3-1
000063145 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000063145 590__ $$a4.122$$b2017
000063145 591__ $$aMULTIDISCIPLINARY SCIENCES$$b12 / 64 = 0.188$$c2017$$dQ1$$eT1
000063145 592__ $$a1.533$$b2017
000063145 593__ $$aMultidisciplinary$$c2017$$dQ1
000063145 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000063145 700__ $$aAyuso, Jose María
000063145 700__ $$aOlave, Marta
000063145 700__ $$0(orcid)0000-0003-0734-129X$$aMonge, Rosa
000063145 700__ $$ade Miguel, Diego
000063145 700__ $$0(orcid)0000-0003-3043-147X$$aMartinez-Lostao, Luis$$uUniversidad de Zaragoza
000063145 700__ $$aLe Gac, Séverine
000063145 700__ $$0(orcid)0000-0001-8741-6452$$aDoblaré, Manuel$$uUniversidad de Zaragoza
000063145 700__ $$0(orcid)0000-0003-2410-5678$$aOchoa, Ignacio$$uUniversidad de Zaragoza
000063145 700__ $$0(orcid)0000-0001-5376-4440$$aFernández, Luis$$uUniversidad de Zaragoza
000063145 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000063145 7102_ $$11008$$2566$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Inmunología
000063145 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000063145 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000063145 773__ $$g7, 1 (2017), 11998 [15 pp]$$pSci. rep.$$tSCIENTIFIC REPORTS$$x2045-2322
000063145 8564_ $$s4700077$$uhttps://zaguan.unizar.es/record/63145/files/texto_completo.pdf$$yVersión publicada
000063145 8564_ $$s107982$$uhttps://zaguan.unizar.es/record/63145/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000063145 909CO $$ooai:zaguan.unizar.es:63145$$particulos$$pdriver
000063145 951__ $$a2020-03-24-10:01:16
000063145 980__ $$aARTICLE