000062009 001__ 62009
000062009 005__ 20190709135504.0
000062009 0247_ $$2doi$$a10.1371/journal.pone.0183416
000062009 0248_ $$2sideral$$a101006
000062009 037__ $$aART-2017-101006
000062009 041__ $$aeng
000062009 100__ $$0(orcid)0000-0003-0582-5418$$aSales Clemente, Ester$$uUniversidad de Zaragoza
000062009 245__ $$aGenome wide association analysis of cold tolerance at germination in temperate japonica rice (Oryza sativa L.) varieties
000062009 260__ $$c2017
000062009 5060_ $$aAccess copy available to the general public$$fUnrestricted
000062009 5203_ $$aA pool of 200 traditional, landraces and modern elite and old cultivars of rice, mainly japonica varieties adapted to temperate regions, have been used to perform a genome wide association study to detect chromosome regions associated to low temperature germination (LTG) regulation using a panel of 1672 SNP markers. Phenotyping was performed by determining growth rates when seeds were germinated at 25Ê and 15ÊC in order to separate the germination vigorousness from cold tolerance effects. As expected, the ability to produce viable seedlings varied widely among rice cultivars and also depended greatly on temperature. Furthermore, we observed a differential response during seed germination and in coleoptile elongation. Faster development at 15ÊC was observed in seeds from varieties traditionally used as cold tolerant parents by breeders, along with other potentially useful cultivars, mainly of Italian origin. When phenotypic data were combined with the panel of SNPs for japonica rice cultivars, significant associations were detected for 31 markers: 7 were related to growth rate at 25ÊC and 24 to growth rates at 15Ê. Among the latter, some chromosome regions were associated to LTG while others were related to coleoptile elongation. Individual effects of the associated markers were low, but by combining favourable alleles in a linear regression model we estimated that 27 loci significantly explained the observed phenotypic variation. From these, a core panel of 13 markers was selected and, furthermore, two wide regions of chromosomes 3 and 6 were consistently associated to rice LTG. Varieties with higher numbers of favourable alleles for the panels of associated markers significantly correlated with increased phenotypic values at both temperatures, thus corroborating the utility of the tagged markers for marker assisted selection (MAS) when breeding japonica rice for LTG.
000062009 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/IPT-2011-1244-010000
000062009 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000062009 590__ $$a2.766$$b2017
000062009 591__ $$aMULTIDISCIPLINARY SCIENCES$$b15 / 64 = 0.234$$c2017$$dQ1$$eT1
000062009 592__ $$a1.164$$b2017
000062009 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2017$$dQ1
000062009 593__ $$aMedicine (miscellaneous)$$c2017$$dQ1
000062009 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2017$$dQ1
000062009 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000062009 700__ $$aViruel Sánchez, Juan
000062009 700__ $$aDomingo Carrasco, Concha
000062009 700__ $$aMarqués Falcó, Luis
000062009 7102_ $$15011$$2705$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Producción Vegetal
000062009 773__ $$g12, e0183416 (2017), [18 pp.]$$pPLoS One$$tPloS one$$x1932-6203
000062009 8564_ $$s2776530$$uhttps://zaguan.unizar.es/record/62009/files/texto_completo.pdf$$yVersión publicada
000062009 8564_ $$s103783$$uhttps://zaguan.unizar.es/record/62009/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000062009 909CO $$ooai:zaguan.unizar.es:62009$$particulos$$pdriver
000062009 951__ $$a2019-07-09-11:47:50
000062009 980__ $$aARTICLE