000061863 001__ 61863
000061863 005__ 20200221144346.0
000061863 0247_ $$2doi$$a10.3109/07420528.2016.1151025
000061863 0248_ $$2sideral$$a94664
000061863 037__ $$aART-2016-94664
000061863 041__ $$aeng
000061863 100__ $$aLax, Pedro
000061863 245__ $$aAge-related changes in photosensitive melanopsin-expressing retinal ganglion cells correlate with circadian rhythm impairments in sighted and blind rats
000061863 260__ $$c2016
000061863 5060_ $$aAccess copy available to the general public$$fUnrestricted
000061863 5203_ $$aThe melanopsin system consists of intrinsically photosensitive retinal ganglion cells containing the photopigment melanopsin (mRGCs). These mRGCs mediate several non-image-forming visual functions, including light entrainment of circadian rhythms. Here we evaluate age-related alterations of the melanopsin system and circadian rhythms in P23H line 1 (P23H-1) rats, a rodent model of retinitis pigmentosa (RP). In homozygous P23H-1 rats and wild-type control rats from the same genetic background (Sprague-Dawley), body temperature and locomotor activity were continuously monitored at 10-min intervals for 7 days, once every 4-5 weeks, between 2 and 24 months of age, using a telemetry transmitter. The distribution and number of mRGCs were assessed in control rats at 12, 18, and 24 months of age and in P23H-1 rats aged 12, 18, 24, and 30 months by immunostaining whole-mount retinas with antibodies against melanopsin. The mean density of mRGCs in control rats showed no significant variations when evaluated at 12 and 18 months of age, and fell by approximately 56% between 18 and 24 months of age. Meanwhile, a significant decrease in the mean number of mRGCs was found in 18-month-old P23H-1 rats as compared to 18-month-old control rats (81% decrease). Parametric and non-parametric analyses of the records showed a gradual age-dependent weakening of body temperature and locomotor activity circadian rhythms robustness in both control and P23H-1 rats from 2 to 24 months of age. However, body temperature and locomotor activity circadian patterns were less robust throughout the experiment in P23H-1 as compared to control rats, with lower amplitude, weaker coupling strength to environmental zeitgebers and higher fragmentation of the rhythms. The present study shows that the degeneration of photoreceptors and inner retinal neurons, characteristic of RP, has age-related degenerative effects on the melanopsin system and is associated with weaker circadian patterns.
000061863 536__ $$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/BFU2012-36845$$9info:eu-repo/grantAgreement/ES/ISCIII/RETICS-RD12-0034-0010$$9info:eu-repo/grantAgreement/ES/ISCIII/PI13-01124
000061863 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000061863 590__ $$a2.562$$b2016
000061863 591__ $$aPHYSIOLOGY$$b35 / 84 = 0.417$$c2016$$dQ2$$eT2
000061863 591__ $$aBIOLOGY$$b23 / 84 = 0.274$$c2016$$dQ2$$eT1
000061863 592__ $$a1.26$$b2016
000061863 593__ $$aPhysiology (medical)$$c2016$$dQ2
000061863 593__ $$aPhysiology$$c2016$$dQ2
000061863 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000061863 700__ $$aEsquiva, Gema
000061863 700__ $$0(orcid)0000-0003-2656-6750$$aFuentes-Broto, Lorena$$uUniversidad de Zaragoza
000061863 700__ $$0(orcid)0000-0002-9250-9060$$aSegura, Francisco$$uUniversidad de Zaragoza
000061863 700__ $$0(orcid)0000-0002-5621-1937$$aSánchez-Cano, Ana$$uUniversidad de Zaragoza
000061863 700__ $$aCuenca, Nicolás
000061863 700__ $$0(orcid)0000-0003-0349-9997$$aPinilla, Isabel$$uUniversidad de Zaragoza
000061863 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología
000061863 7102_ $$11004$$2646$$aUniversidad de Zaragoza$$bDpto. Cirugía,Ginecol.Obstetr.$$cÁrea Oftalmología
000061863 7102_ $$12002$$2647$$aUniversidad de Zaragoza$$bDpto. Física Aplicada$$cÁrea Óptica
000061863 773__ $$g33, 4 (2016), [18 pp.]$$pChronobiol. int.$$tCHRONOBIOLOGY INTERNATIONAL$$x0742-0528
000061863 8564_ $$s2179917$$uhttps://zaguan.unizar.es/record/61863/files/texto_completo.pdf$$yPostprint
000061863 8564_ $$s115934$$uhttps://zaguan.unizar.es/record/61863/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000061863 909CO $$ooai:zaguan.unizar.es:61863$$particulos$$pdriver
000061863 951__ $$a2020-02-21-13:52:48
000061863 980__ $$aARTICLE