Atlantis Institut des Sciences Fictives

Resumen: Background: Oxidative stress plays a key role in the development of intestinal inflammatory diseases. Several pro-inflammatory mediators may generate oxidation products that exacerbate the inflammatory damage. Gastrointestinal molecules, like serotonin (5- HT), adenosine and melatonin, which are involved in intestinal physiology, have also been described as intestinal pro-inflammatory factors; whereas IL-10, a known anti-inflammatory cytokine, has also been implicated in intestinal pathophysiology. Aim: The aim of this study was to analyze the contribution of pro-inflammatory and anti-inflammatory molecules to oxidative stress balance, as well as to assess their effect on cellular antioxidant enzymes activity, in intestinal epithelial cells. Methods: Caco-2 cells were treated with the different molecules, and the oxidative stress was determined by measuring lipid peroxidation (MDA+4HDA) and protein carbonyl levels. The activity of the anti-oxidant enzymes (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) was also analyzed in treated cells. Results: Pro-inflammatory factors (5-HT, adenosine, melatonin and TNFa) increased oxidative damage in both lipids and proteins. Theses molecules, except melatonin, also inhibited the activity of antioxidant enzymes. With regard to IL-10, this cytokine was not shown to alter cellular oxidative damage, but was able to reduce the oxidative damage g by pro-inflamatory factors, and to restore their effects on anti-oxidant enxymes activities. Unexpectedly, IL-10, together with melatonin, was found to increase the antioxidant activity above the control. Conclusions: The anti-oxidant effect of IL-10 emphasizes the role of this cytokine as a potential therapy for the treatment of intestinal inflammation induced by pro-inflammatory molecules.
Idioma: Inglés
DOI: 10.1111/apha.12498
Año: 2015
Publicado en: Acta Physiologica 214, Suppl. 701 (2015), 30 [14]
ISSN: 1748-1708
Factor impacto JCR: 4.066 (2015)
Categ. JCR: PHYSIOLOGY rank: 13 / 83 = 0.157 (2015) - Q1 - T1
Factor impacto SCIMAGO: 1.654 - Physiology (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/ARAINF-012-2008
Financiación: info:eu-repo/grantAgreement/ES/DGA/B022-13
Financiación: info:eu-repo/grantAgreement/ES/DGA/B105-11
Financiación: info:eu-repo/grantAgreement/ES/DGA/B61
Financiación: info:eu-repo/grantAgreement/ES/MICINN-FEDER/BFU2009-08149
Financiación: info:eu-repo/grantAgreement/ES/MICINN-FEDER/BFU2010-18971
Tipo y forma: Article (Published version)
Área (Departamento): Área Fisiología (Dpto. Farmacología y Fisiolog.)
Área (Departamento): Area Medicina (Dpto. Medicina, Psiqu. y Derm.)
Exportado de SIDERAL (2024-01-22-15:31:36)


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