000061493 001__ 61493
000061493 005__ 20190529115218.0
000061493 0247_ $$2doi$$a10.1371/journal.pone.0065243
000061493 0248_ $$2sideral$$a83186
000061493 037__ $$aART-2013-83186
000061493 041__ $$aeng
000061493 100__ $$aCabezas-Cruz, A.
000061493 245__ $$aFunctional and Immunological Relevance of Anaplasma marginale Major Surface Protein 1a Sequence and Structural Analysis
000061493 260__ $$c2013
000061493 5060_ $$aAccess copy available to the general public$$fUnrestricted
000061493 5203_ $$aBovine anaplasmosis is caused by cattle infection with the tick-borne bacterium, Anaplasma marginale. The major surface protein 1a (MSP1a) has been used as a genetic marker for identifying A. marginale strains based on N-terminal tandem repeats and a 5'-UTR microsatellite located in the msp1a gene. The MSP1a tandem repeats contain immune relevant elements and functional domains that bind to bovine erythrocytes and tick cells, thus providing information about the evolution of host-pathogen and vector-pathogen interactions. Here we propose one nomenclature for A. marginale strain classification based on MSP1a. All tandem repeats among A. marginale strains were classified and the amino acid variability/frequency in each position was determined. The sequence variation at immunodominant B cell epitopes was determined and the secondary (2D) structure of the tandem repeats was modeled. A total of 224 different strains of A. marginale were classified, showing 11 genotypes based on the 5'-UTR microsatellite and 193 different tandem repeats with high amino acid variability per position. Our results showed phylogenetic correlation between MSP1a sequence, secondary structure, B-cell epitope composition and tick transmissibility of A. marginale strains. The analysis of MSP1a sequences provides relevant information about the biology of A. marginale to design vaccines with a cross-protective capacity based on MSP1a B-cell epitopes.
000061493 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/BFU2011-23896$$9info:eu-repo/grantAgreement/EC/FP7/238511/EU/Post-graduate training network for capacity building to control ticks and tick-borne diseases/POSTICK
000061493 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000061493 590__ $$a3.534$$b2013
000061493 591__ $$aMULTIDISCIPLINARY SCIENCES$$b8 / 56 = 0.143$$c2013$$dQ1$$eT1
000061493 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000061493 700__ $$aPassos, L.M.F.
000061493 700__ $$aLis, K.
000061493 700__ $$aKenneil, R.
000061493 700__ $$aValdés, J.J.
000061493 700__ $$aFerrolho, J.
000061493 700__ $$aTonk, M.
000061493 700__ $$aPohl, A.E.
000061493 700__ $$aGrubhoffer, L.
000061493 700__ $$aZweygarth, E.
000061493 700__ $$aShkap, V.
000061493 700__ $$aRibeiro, M.F.B.
000061493 700__ $$0(orcid)0000-0001-7483-046X$$aEstrada-Peña, A.$$uUniversidad de Zaragoza
000061493 700__ $$aKocan, K.M.
000061493 700__ $$ade la Fuente, J.
000061493 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000061493 773__ $$g8, 6 (2013), e65243 [13 pp]$$pPLoS One$$tPLoS ONE$$x1932-6203
000061493 8564_ $$s4270454$$uhttp://zaguan.unizar.es/record/61493/files/texto_completo.pdf$$yVersión publicada
000061493 8564_ $$s125430$$uhttp://zaguan.unizar.es/record/61493/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000061493 909CO $$ooai:zaguan.unizar.es:61493$$particulos$$pdriver
000061493 951__ $$a2019-05-29-11:40:58
000061493 980__ $$aARTICLE