000061469 001__ 61469
000061469 005__ 20200428120340.0
000061469 0247_ $$2doi$$a10.9755/ejfa.2016-11-1585
000061469 0248_ $$2sideral$$a98854
000061469 037__ $$aART-2017-98854
000061469 041__ $$aeng
000061469 100__ $$aFatnassi, Meriem
000061469 245__ $$aIdentification of ß-nerve growth factor in dromedary camel seminal plasma and its role in induction of ovulation in females
000061469 260__ $$c2017
000061469 5060_ $$aAccess copy available to the general public$$fUnrestricted
000061469 5203_ $$aThe main objective of this study was to demonstrate the effect of seminal plasma ovulation inducing factor (OIF) on ovulation in female camels. Seminal plasma was fractionated using gel filtration chromatography, and two protein peaks were obtained and analysed by western blotting. The effect of the bioactive protein fraction (OIF/ß-NGF) was tested by intramuscular injection (1 ml) in synchronized females with the following treatments: PBS (negative control group, n=3); 20 /jg Buserelin (GnRH analogue, positive control group; n=3); and purified OIF with doses of 1 and 2 mg (n = 5, each treatment). Blood samples were collected every two days from day 0 until 14 days post-treatment, and the progesterone concentration was assessed. The obtained results showed that the OIF is highly present in the seminal plasma of dromedary camels as a protein with a molecular mass of approximately 14 kDa. It was detected as a betaNerve Growth Factor, named Cam-ß-NGF. The effect of this molecule on ovulation was clearly demonstrated by the significant increase of the plasma progesterone concentration in the treated female groups (1 and 2 mg of Cam-ß-NGF) as observed in Buserelin group. In summary, intramuscular injection of ß-NGF isolated from dromedary camel seminal plasma induces ovulation in females with similar rate to Buserelin treatment.
000061469 536__ $$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL2013-43328-P$$9info:eu-repo/grantAgreement/ES/DGA/A26
000061469 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000061469 590__ $$a0.609$$b2017
000061469 591__ $$aAGRONOMY$$b66 / 87 = 0.759$$c2017$$dQ4$$eT3
000061469 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b110 / 133 = 0.827$$c2017$$dQ4$$eT3
000061469 592__ $$a0.27$$b2017
000061469 593__ $$aAgronomy and Crop Science$$c2017$$dQ3
000061469 593__ $$aFood Science$$c2017$$dQ3
000061469 593__ $$aApplied Microbiology and Biotechnology$$c2017$$dQ3
000061469 593__ $$aAnimal Science and Zoology$$c2017$$dQ3
000061469 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000061469 700__ $$0(orcid)0000-0001-9842-6197$$aCebrián-Pérez, José Alvaro$$uUniversidad de Zaragoza
000061469 700__ $$aSalhi, Imed
000061469 700__ $$0(orcid)0000-0002-2312-6402$$aPérez-Pé, Rosaura$$uUniversidad de Zaragoza
000061469 700__ $$aSeddik, Mabrouk Mouldi
000061469 700__ $$0(orcid)0000-0003-1997-4262$$aCasao, Adriana$$uUniversidad de Zaragoza
000061469 700__ $$aKhorchani, Touhami
000061469 700__ $$0(orcid)0000-0003-1306-8517$$aMuiño-Blanco, Teresa$$uUniversidad de Zaragoza
000061469 700__ $$aHammadi, Mohamed
000061469 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000061469 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000061469 773__ $$g29, 4 (2017), 293-299$$pEmir. j. food agric.$$tEmirates Journal of Food and Agriculture$$x2079-052X
000061469 8564_ $$s739188$$uhttps://zaguan.unizar.es/record/61469/files/texto_completo.pdf$$yVersión publicada
000061469 8564_ $$s111924$$uhttps://zaguan.unizar.es/record/61469/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000061469 909CO $$ooai:zaguan.unizar.es:61469$$particulos$$pdriver
000061469 951__ $$a2020-04-28-12:01:09
000061469 980__ $$aARTICLE