000061465 001__ 61465
000061465 005__ 20170612112602.0
000061465 0247_ $$2doi$$a10.1128/AEM.01168-13
000061465 0248_ $$2sideral$$a83054
000061465 037__ $$aART-2013-83054
000061465 041__ $$aeng
000061465 100__ $$0(orcid)0000-0001-7831-2483$$aQuílez, J.$$uUniversidad de Zaragoza
000061465 245__ $$aHost association of cryptosporidium parvum populations infecting domestic ruminants in Spain
000061465 260__ $$c2013
000061465 5060_ $$aAccess copy available to the general public$$fUnrestricted
000061465 5203_ $$aA stock of 148 Cryptosporidium parvum DNA extracts from lambs and goat kids selected from a previous study examining the occurrence of Cryptosporidium species and GP60 subtypes in diarrheic lambs and goat kids in northeastern Spain was further characterized by a multilocus fragment typing approach with six mini- and microsatellite loci. Various degrees of polymorphism were seen at all but the MS5 locus, although all markers exhibited two major alleles accounting for more than 75% of isolates. A total of 56 multilocus subtypes (MLTs) from lambs (48 MLTs) and goat kids (11 MLTs) were identified. Individual isolates with mixed MLTs were detected on more than 25% of the farms, but most MLTs (33) were distinctive for individual farms, revealing the endemicity of cryptosporidial infections on sheep and goat farms. Comparison with a previous study in calves in northern Spain using the same six-locus subtyping scheme showed the presence of host-associated alleles, differences in the identity of major alleles, and very little overlap in MLTs between C. parvum isolates from lambs and those from calves (1 MLT) or isolates from lambs and those from goat kids (3 MLTs). The Hunter-Gaston index of the multilocus technique was 0.976 (95% confidence interval [CI], 0.970 to 0.982), which supports its high discriminatory power for strain typing and epidemiological tracking. Population analyses revealed the presence of two host-associated subpopulations showing epidemic clonality among the C. parvum isolates infecting calves and lambs/goat kids, respectively, although evidence of genetic flow between the two subpopulations was also detected.
000061465 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B82$$9info:eu-repo/grantAgreement/ES/MICINN/AGL2009-10590
000061465 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000061465 590__ $$a3.952$$b2013
000061465 591__ $$aMICROBIOLOGY$$b24 / 118 = 0.203$$c2013$$dQ1$$eT1
000061465 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b30 / 161 = 0.186$$c2013$$dQ1$$eT1
000061465 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000061465 700__ $$0(orcid)0000-0002-9243-7503$$aVergara Castiblanco, C.
000061465 700__ $$0(orcid)0000-0003-0210-2919$$aMonteagudo, L.$$uUniversidad de Zaragoza
000061465 700__ $$0(orcid)0000-0002-9453-7655$$aCacho, E.$$uUniversidad de Zaragoza
000061465 700__ $$0(orcid)0000-0002-3532-0384$$aSánchez Acedo, C.$$uUniversidad de Zaragoza
000061465 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDepartamento de Patología Animal$$cSanidad Animal
000061465 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDepartamento de Anatomía, Embriología y Genética Animal$$cGenética
000061465 773__ $$g79, 17 (2013), 5363-5371$$pAppl. environ. microbiol.$$tAPPLIED AND ENVIRONMENTAL MICROBIOLOGY$$x0099-2240
000061465 8564_ $$s878807$$uhttp://zaguan.unizar.es/record/61465/files/texto_completo.pdf$$yVersión publicada
000061465 8564_ $$s138245$$uhttp://zaguan.unizar.es/record/61465/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000061465 909CO $$ooai:zaguan.unizar.es:61465$$particulos$$pdriver
000061465 951__ $$a2017-06-12-09:14:48
000061465 980__ $$aARTICLE