000061319 001__ 61319
000061319 005__ 20200221144325.0
000061319 0247_ $$2doi$$a10.1128/AEM.03761-15
000061319 0248_ $$2sideral$$a94690
000061319 037__ $$aART-2016-94690
000061319 041__ $$aeng
000061319 100__ $$aMathieu,Yann
000061319 245__ $$aActivities of secreted aryl alcohol quinone oxidoreductases from Pycnoporus cinnabarinus provide insights into fungal degradation of plant biomass
000061319 260__ $$c2016
000061319 5060_ $$aAccess copy available to the general public$$fUnrestricted
000061319 5203_ $$aAuxiliary activities family 3 subfamily 2 (AA3_2) from the CAZy database comprises various functions related to ligninolytic enzymes, such as fungal aryl alcohol oxidases (AAO) and glucose oxidases, both of which are flavoenzymes. The recent study of the Pycnoporus cinnabarinus CIRM BRFM 137 genome combined with its secretome revealed that four AA3_2 enzymes are secreted during biomass degradation. One of these AA3_2 enzymes, scf184803.g17, has recently been produced heterologously in Aspergillus niger. Based on the enzyme''s activity and specificity, it was assigned to the glucose dehydrogenases (P. cinnabarinus GDH PcGDH]). Here, we analyze the distribution of the other three AA3_2 enzymes (scf185002.g8, scf184611.g7, and scf184746.g13) to assess their putative functions. These proteins showed the highest homology with aryl alcohol oxidase from Pleurotus eryngii. Biochemical characterization demonstrated that they were also flavoenzymes harboring flavin adenine dinucleotide (FAD) as a cofactor and able to oxidize a wide variety of phenolic and nonphenolic aryl alcohols and one aliphatic polyunsaturated primary alcohol. Though presenting homology with fungal AAOs, these enzymes exhibited greater efficiency in reducing electron acceptors (quinones and one artificial acceptor) than molecular oxygen and so were defined as aryl-alcohol: quinone oxidoreductases (AAQOs) with two enzymes possessing residual oxidase activity (PcAAQO2 and PcAAQO3). Structural comparison of PcAAQO homology models with P. eryngii AAO demonstrated a wider substrate access channel connecting the active-site cavity to the solvent, explaining the absence of activity with molecular oxygen. Finally, the ability of PcAAQOs to reduce radical intermediates generated by laccase from P. cinnabarinus was demonstrated, shedding light on the ligninolytic system of this fungus.
000061319 536__ $$9info:eu-repo/grantAgreement/EC/FP7/613549/EU/Optimized oxidoreductases for medium and large scale industrial biotransformations/INDOX
000061319 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000061319 590__ $$a3.807$$b2016
000061319 591__ $$aMICROBIOLOGY$$b28 / 124 = 0.226$$c2016$$dQ1$$eT1
000061319 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b33 / 160 = 0.206$$c2016$$dQ1$$eT1
000061319 592__ $$a1.705$$b2016
000061319 593__ $$aApplied Microbiology and Biotechnology$$c2016$$dQ1
000061319 593__ $$aFood Science$$c2016$$dQ1
000061319 593__ $$aEcology$$c2016$$dQ1
000061319 593__ $$aBiotechnology$$c2016$$dQ1
000061319 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000061319 700__ $$aPiumi,Francois
000061319 700__ $$aValli,Richard
000061319 700__ $$aCarro Aramburu,Juan
000061319 700__ $$0(orcid)0000-0003-4076-6118$$aFerreira,Patricia$$uUniversidad de Zaragoza
000061319 700__ $$aFaulds,Craig B.
000061319 700__ $$aRecord,Eric
000061319 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000061319 773__ $$g82, 8 (2016), 2411-2423$$pAppl. environ. microbiol.$$tApplied and Environmental Microbiology$$x0099-2240
000061319 85641 $$uhttps://aem.asm.org/content/aem/82/8/2411.full.pdf$$zTexto completo de la revista
000061319 8564_ $$s2580828$$uhttps://zaguan.unizar.es/record/61319/files/texto_completo.pdf$$yVersión publicada
000061319 8564_ $$s136611$$uhttps://zaguan.unizar.es/record/61319/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000061319 909CO $$ooai:zaguan.unizar.es:61319$$particulos$$pdriver
000061319 951__ $$a2020-02-21-13:43:43
000061319 980__ $$aARTICLE