000060990 001__ 60990
000060990 005__ 20190709135530.0
000060990 0247_ $$2doi$$a10.3390/ijms18030591
000060990 0248_ $$2sideral$$a98629
000060990 037__ $$aART-2017-98629
000060990 041__ $$aeng
000060990 100__ $$aZaouali, M. A.
000060990 245__ $$aGSK3ß and VDAC involvement in ER stress and apoptosis modulation during orthotopic liver transplantation
000060990 260__ $$c2017
000060990 5060_ $$aAccess copy available to the general public$$fUnrestricted
000060990 5203_ $$aWe investigated the involvement of glycogen synthase kinase-3ß (GSK3ß) and the voltage-dependent anion channel (VDAC) in livers subjected to cold ischemia–reperfusion injury (I/R) associated with orthotopic liver transplantation (OLT). Rat livers were preserved in University of Wisconsin (UW) and Institute Georges Lopez (IGL-1) solution, the latter enriched or not with trimetazidine, and then subjected to OLT. Transaminase (ALT) and HMGB1 protein levels, glutamate dehydrogenase (GLDH), and oxidative stress (MDA) were measured. The AKT protein kinase and its direct substrates, GSK3ß and VDAC, as well as caspases 3, 9, and cytochrome C and reticulum endoplasmic stress-related proteins (GRP78, pPERK, ATF4, and CHOP), were determined by Western blot. IGL-1+TMZ significantly reduced liver injury. We also observed a significant phosphorylation of AKT, which in turn induced the phosphorylation and inhibition of GSK3ß. In addition, TMZ protected the mitochondria since, in comparison with IGL-1 alone, we found reductions in VDAC phosphorylation, apoptosis, and GLDH release. All these results were correlated with decreased ER stress. Addition of TMZ to IGL-1 solution increased the tolerance of the liver graft to I/R injury through inhibition of GSK3ß and VDAC, contributing to ER stress reduction and cell death prevention.
000060990 536__ $$9info:eu-repo/grantAgreement/ES/FIS/PI12-0056
000060990 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000060990 590__ $$a3.687$$b2017
000060990 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b52 / 171 = 0.304$$c2017$$dQ2$$eT1
000060990 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b90 / 292 = 0.308$$c2017$$dQ2$$eT1
000060990 592__ $$a1.26$$b2017
000060990 593__ $$aMedicine (miscellaneous)$$c2017$$dQ1
000060990 593__ $$aPhysical and Theoretical Chemistry$$c2017$$dQ1
000060990 593__ $$aComputer Science Applications$$c2017$$dQ1
000060990 593__ $$aInorganic Chemistry$$c2017$$dQ1
000060990 593__ $$aSpectroscopy$$c2017$$dQ1
000060990 593__ $$aOrganic Chemistry$$c2017$$dQ1
000060990 593__ $$aMolecular Biology$$c2017$$dQ2
000060990 593__ $$aCatalysis$$c2017$$dQ2
000060990 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000060990 700__ $$aPanisello, A.
000060990 700__ $$aLopez, A.
000060990 700__ $$aCastro, C.
000060990 700__ $$aFolch, E.
000060990 700__ $$aCarbonell, T.
000060990 700__ $$aRolo, A.
000060990 700__ $$aPalmeira, C. M.
000060990 700__ $$0(orcid)0000-0002-8275-7191$$aGarcia-Gil, A.$$uUniversidad de Zaragoza
000060990 700__ $$aAdam, R.
000060990 700__ $$aRoselló-Catafau, J.
000060990 7102_ $$11004$$2090$$aUniversidad de Zaragoza$$bDpto. Cirugía,Ginecol.Obstetr.$$cÁrea Cirugía
000060990 773__ $$g18, 3 (2017), 591 [15 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000060990 8564_ $$s13013031$$uhttps://zaguan.unizar.es/record/60990/files/texto_completo.pdf$$yVersión publicada
000060990 8564_ $$s103213$$uhttps://zaguan.unizar.es/record/60990/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000060990 909CO $$ooai:zaguan.unizar.es:60990$$particulos$$pdriver
000060990 951__ $$a2019-07-09-12:02:08
000060990 980__ $$aARTICLE