000060968 001__ 60968 000060968 005__ 20240118091946.0 000060968 0247_ $$2doi$$a10.3390/ijms18030662 000060968 0248_ $$2sideral$$a98505 000060968 037__ $$aART-2017-98505 000060968 041__ $$aeng 000060968 100__ $$0(orcid)0000-0003-2745-4152$$aGonzález-Arto, M. 000060968 245__ $$aMelatonin MT1 and MT2 receptors in the ram reproductive tract 000060968 260__ $$c2017 000060968 5060_ $$aAccess copy available to the general public$$fUnrestricted 000060968 5203_ $$aSome melatonin functions in mammals are exerted through MT1 and MT2 receptors. However, there are no reports of their presence in the reproductive tract of the ram, a seasonal species. Thus, we have investigated their existence in the ram testis, epididymis, accessory glands and ductus deferens. Real-time polymerase chain reaction (qPCR) revealed higher levels of m-RNA for both receptors in the testis, ampulla, seminal vesicles, and vas deferens, than in the other organs of the reproductive tract (p < 0.05). Western blot analyses showed protein bands compatible with the MT1 in the testis and cauda epididymis, and for the MT2 in the cauda epididymis and deferent duct. Immunohistochemistry analyses revealed the presence of MT1 receptors in spermatogonias, spermatocytes, and spermatids, and MT2 receptors in the newly-formed spermatozoa in the testis, whereas both receptors were located in the epithelial cells of the ampulla, seminal vesicles, and ductus deferens. Indirect immunofluorescence showed significant differences in the immunolocation of both receptors in spermatozoa during their transit in the epididymis. In conclusion, it was demonstrated that melatonin receptors are present in the ram reproductive tract. These results open the way for new studies on the molecular mechanism of melatonin and the biological significance of its receptors. 000060968 536__ $$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL2013-43328-P$$9info:eu-repo/grantAgreement/ES/DGA/A26 000060968 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000060968 590__ $$a3.687$$b2017 000060968 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b52 / 169 = 0.308$$c2017$$dQ2$$eT1 000060968 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b90 / 292 = 0.308$$c2017$$dQ2$$eT1 000060968 592__ $$a1.26$$b2017 000060968 593__ $$aMedicine (miscellaneous)$$c2017$$dQ1 000060968 593__ $$aPhysical and Theoretical Chemistry$$c2017$$dQ1 000060968 593__ $$aComputer Science Applications$$c2017$$dQ1 000060968 593__ $$aInorganic Chemistry$$c2017$$dQ1 000060968 593__ $$aSpectroscopy$$c2017$$dQ1 000060968 593__ $$aOrganic Chemistry$$c2017$$dQ1 000060968 593__ $$aMolecular Biology$$c2017$$dQ2 000060968 593__ $$aCatalysis$$c2017$$dQ2 000060968 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000060968 700__ $$0(orcid)0000-0001-5940-3339$$aAguilar, D. 000060968 700__ $$aGaspar-Torrubia, E. 000060968 700__ $$0(orcid)0000-0003-3945-4854$$aGallego, M.$$uUniversidad de Zaragoza 000060968 700__ $$0(orcid)0000-0003-3929-5064$$aCarvajal-Serna, M. 000060968 700__ $$0(orcid)0000-0002-4665-9674$$aHerrera-Marcos, L.$$uUniversidad de Zaragoza 000060968 700__ $$0(orcid)0000-0001-5276-7834$$aSerrano-Blesa, E. 000060968 700__ $$aDos, Santos Hamilton 000060968 700__ $$0(orcid)0000-0002-2312-6402$$aPérez-Pé, R.$$uUniversidad de Zaragoza 000060968 700__ $$0(orcid)0000-0003-1306-8517$$aMuiño-Blanco, T.$$uUniversidad de Zaragoza 000060968 700__ $$0(orcid)0000-0001-9842-6197$$aCebrián-Pérez, J. A.$$uUniversidad de Zaragoza 000060968 700__ $$0(orcid)0000-0003-1997-4262$$aCasao, A.$$uUniversidad de Zaragoza 000060968 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología 000060968 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000060968 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole. 000060968 773__ $$g18, 3 (2017), 662 [15 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596 000060968 8564_ $$s8405029$$uhttps://zaguan.unizar.es/record/60968/files/texto_completo.pdf$$yVersión publicada 000060968 8564_ $$s100913$$uhttps://zaguan.unizar.es/record/60968/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000060968 909CO $$ooai:zaguan.unizar.es:60968$$particulos$$pdriver 000060968 951__ $$a2024-01-18-09:10:25 000060968 980__ $$aARTICLE