000060883 001__ 60883
000060883 005__ 20170503142843.0
000060883 0247_ $$2doi$$a10.1128/JCM.02099-12
000060883 0248_ $$2sideral$$a81810
000060883 037__ $$aART-2013-81810
000060883 041__ $$aeng
000060883 100__ $$0(orcid)0000-0001-5442-7702$$aMainar-Jaime, R.$$uUniversidad de Zaragoza
000060883 245__ $$aSensitivity of the ISO 6579:2002/Amd 1:2007 standard method for detection of Salmonella spp. on mesenteric lymph nodes from slaughter pigs
000060883 260__ $$c2013
000060883 5060_ $$aAccess copy available to the general public$$fUnrestricted
000060883 5203_ $$aThe ISO 6579:2002/Amd 1:2007 (ISO) standard has been the bacteriological standard method used in the European Union for the detection of Salmonella spp. in pig mesenteric lymph nodes (MLN), but there are no published estimates of the diagnostic sensitivity (Se) of the method in this matrix. Here, the Se of the ISO (SeISO) was estimated on 675 samples selected from two populations with different Salmonella prevalences (14 farms with a =20% prevalence and 13 farms with a <20% prevalence) and through the use of latent-class models in concert with Bayesian inference, assuming 100% ISO specificity, and an invA-based PCR as the second diagnostic method. The SeISO was estimated to be close to 87%, while the sensitivity of the PCR reached up to 83.6% and its specificity was 97.4%. Interestingly, the bacteriological reanalysis of 33 potential false-negative (PCR-positive) samples allowed isolation of 19 (57.5%) new Salmonella strains, improving the overall diagnostic accuracy of the bacteriology. Considering the usual limitations of bacteriology regarding Se, these results support the adequacy of the ISO for the detection of Salmonella spp. from MLN and also that of the PCR-based method as an alternative or complementary (screening) test for the diagnosis of pig salmonellosis, particularly considering the cost and time benefits of the molecular procedure.
000060883 536__ $$9info:eu-repo/grantAgreement/ES/INIA/FAU2008-16$$9info:eu-repo/grantAgreement/ES/INIA/RTA2007-65
000060883 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000060883 590__ $$a4.232$$b2013
000060883 591__ $$aMICROBIOLOGY$$b23 / 118 = 0.195$$c2013$$dQ1$$eT1
000060883 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000060883 700__ $$aAndrés, S.
000060883 700__ $$0(orcid)0000-0001-5313-7033$$aVico, J.P.
000060883 700__ $$aSan Román, B.
000060883 700__ $$aGarrido, V.
000060883 700__ $$aGrilló, M.J.
000060883 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDepartamento de Patología Animal$$cSanidad Animal
000060883 773__ $$g51, 1 (2013), 89-94$$pJ. clin. microbiol.$$tJOURNAL OF CLINICAL MICROBIOLOGY$$x0095-1137
000060883 8564_ $$s162123$$uhttp://zaguan.unizar.es/record/60883/files/texto_completo.pdf$$yVersión publicada
000060883 8564_ $$s132546$$uhttp://zaguan.unizar.es/record/60883/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000060883 909CO $$ooai:zaguan.unizar.es:60883$$particulos$$pdriver
000060883 951__ $$a2017-05-03-14:22:46
000060883 980__ $$aARTICLE