000060880 001__ 60880
000060880 005__ 20170503142843.0
000060880 0247_ $$2doi$$a10.1128/JCM.01944-12
000060880 0248_ $$2sideral$$a82939
000060880 037__ $$aART-2013-82939
000060880 041__ $$aeng
000060880 100__ $$aVillellas, C.
000060880 245__ $$aAnalysis of mutations in streptomycin-resistant strains reveals a simple and reliable genetic marker for identification of the Mycobacterium tuberculosis Beijing genotype
000060880 260__ $$c2013
000060880 5060_ $$aAccess copy available to the general public$$fUnrestricted
000060880 5203_ $$aThe Mycobacterium tuberculosis pandemic is a major health problem, further complicated by an increasing incidence of drug-resistant isolates and the existence of highly transmissible strains, such as those in the Beijing family. Streptomycin (STR)-resistant M. tuberculosis clinical isolates have been analyzed to look for mutations in the rpsL, rrs, and gidB genes. In addition, the Rv1258c gene, which encodes Tap, an efflux pump that transports STR, has been sequenced. Mutations affecting codons 43 and 88 of the rpsL gene were found in 44.4% of the strains, and 16.7% of the strains carried mutations in the rrs gene, both of which probably contribute to STR resistance. Many strains presented with mutations in the gidB gene, but the implication of those mutations in STR resistance remains unclear. Interestingly, a cytosine nucleotide insertion between positions 580 and 581 (denominated Tap580) in the Rv1258c gene has been found in all Beijing isolates included in this study, suggesting that it might be a novel polymorphism specific to the Beijing family of M. tuberculosis. A simple and fast restriction fragment length polymorphism (RFLP)-PCR method for detecting the Tap580 insertion has been developed and used to screen a collection of 220 DNA samples obtained from cultures of M. tuberculosis isolates and 30 respiratory specimens. In all cases, the Beijing and non-Beijing representative samples were identified correctly. Tap580 is a novel polymorphism specific to the highly transmissible Beijing family, which allows for fast detection of these strains even at the very early stages of infection.
000060880 536__ $$9info:eu-repo/grantAgreement/ES/MEC/FPU-AP2008-04730$$9info:eu-repo/grantAgreement/ES/MICINN/BIO2009-09405
000060880 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000060880 590__ $$a4.232$$b2013
000060880 591__ $$aMICROBIOLOGY$$b23 / 118 = 0.195$$c2013$$dQ1$$eT1
000060880 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000060880 700__ $$aAristimuño, L.
000060880 700__ $$0(orcid)0000-0003-4198-6622$$aVitoria, M.A.$$uUniversidad de Zaragoza
000060880 700__ $$aPrat, C.
000060880 700__ $$aBlanco, S.
000060880 700__ $$aDe Viedma, D.G.
000060880 700__ $$aDomínguez, J.
000060880 700__ $$0(orcid)0000-0002-3011-2519$$aSamper, S.
000060880 700__ $$0(orcid)0000-0003-2076-844X$$aAínsa, J.A.$$uUniversidad de Zaragoza
000060880 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDepartamento de Microbiología, Medicina Preventiva y Salud Pública$$cMicrobiología
000060880 773__ $$g51, 7 (2013), 2124-2130$$pJ. clin. microbiol.$$tJOURNAL OF CLINICAL MICROBIOLOGY$$x0095-1137
000060880 8564_ $$s496929$$uhttp://zaguan.unizar.es/record/60880/files/texto_completo.pdf$$yVersión publicada
000060880 8564_ $$s134640$$uhttp://zaguan.unizar.es/record/60880/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000060880 909CO $$ooai:zaguan.unizar.es:60880$$particulos$$pdriver
000060880 951__ $$a2017-05-03-14:22:48
000060880 980__ $$aARTICLE