000060879 001__ 60879
000060879 005__ 20170503142843.0
000060879 0247_ $$2doi$$a10.1128/JCM.00563-14
000060879 0248_ $$2sideral$$a87616
000060879 037__ $$aART-2014-87616
000060879 041__ $$aeng
000060879 100__ $$aAnneloes, V.
000060879 245__ $$aInterlaboratory comparison of sample preparation methods, database expansions, and cutoff values for identification of yeasts by matrix-assisted laser desorption ionization-time of flight mass spectrometry using a yeast test panel
000060879 260__ $$c2014
000060879 5060_ $$aAccess copy available to the general public$$fUnrestricted
000060879 5203_ $$aAn interlaboratory study using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) to determine the identification of clinically important yeasts (n35) was performed at 11 clinical centers, one company, and one reference center using the Bruker Daltonics MALDI Biotyper system. The optimal cutoff for the MALDI-TOF MS score was investigated using receiver operating characteristic (ROC) curve analyses. The percentages of correct identifications were compared for different sample preparation methods and different databases. Logistic regression analysis was performed to analyze the association between the number of spectra in the database and the percentage of strains that were correctly identified. A total of 5,460 MALDI-TOF MS results were obtained. Using all results, the area under the ROC curve was 0.95 (95% confidence interval [CI], 0.94 to 0.96). With a sensitivity of 0.84 and a specificity of 0.97, a cutoff value of 1.7 was considered optimal. The overall percentage of correct identifications (formic acid-ethanol extraction method, score>1.7) was 61.5% when the commercial Bruker Daltonics database (BDAL) was used, and it increased to 86.8% by using an extended BDAL supplemented with a Centraalbureau voor Schimmelcultures (CBS)-KNAW Fungal Biodiversity Centre in-house database (BDALCBS in-house). A greater number of main spectra (MSP) in the database was associated with a higher percentage of correct identifications (odds ratio [OR], 1.10; 95% CI, 1.05 to 1.15; P<0.01). The results from the direct transfer method ranged from 0% to 82.9% correct identifications, with the results of the top four centers ranging from 71.4% to 82.9% correct identifications. This study supports the use of a cutoff value of 1.7 for the identification of yeasts using MALDI-TOF MS. The inclusion of enough isolates of the same species in the database can enhance the proportion of correctly identified strains. Further optimization of the preparation methods, especially of the direct transfer method, may contribute to improved diagnosis of yeast-related infections.
000060879 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000060879 590__ $$a3.993$$b2014
000060879 591__ $$aMICROBIOLOGY$$b26 / 119 = 0.218$$c2014$$dQ1$$eT1
000060879 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000060879 700__ $$aKolecka, A.
000060879 700__ $$aKhayhan, K.
000060879 700__ $$aTheelen, B.
000060879 700__ $$aGroenewald, M.
000060879 700__ $$aBoel, E.
000060879 700__ $$aBoekhout, T.
000060879 700__ $$aJansz, A.
000060879 700__ $$aDe Koning, B.
000060879 700__ $$aFaro, D.
000060879 700__ $$aGarcía-Rodríguez, J.
000060879 700__ $$aKampinga, G.A.
000060879 700__ $$aKostrzewa, M.
000060879 700__ $$aKuijper, E.J.
000060879 700__ $$aKusters, J.G.
000060879 700__ $$aLass-Flörl, C.
000060879 700__ $$aMeis, J.F.
000060879 700__ $$aMertens, A.
000060879 700__ $$aPeterse, N.
000060879 700__ $$0(orcid)0000-0001-7294-245X$$aRezusta, A.$$uUniversidad de Zaragoza
000060879 700__ $$aVan Herendael, B.
000060879 700__ $$aVan Marm, S.
000060879 700__ $$aWunderink, H.F.
000060879 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDepartamento de Microbiología, Medicina Preventiva y Salud Pública$$cMicrobiología
000060879 773__ $$g52, 8 (2014), 3023-3029$$pJ. clin. microbiol.$$tJOURNAL OF CLINICAL MICROBIOLOGY$$x0095-1137
000060879 8564_ $$s163961$$uhttp://zaguan.unizar.es/record/60879/files/texto_completo.pdf$$yVersión publicada
000060879 8564_ $$s132067$$uhttp://zaguan.unizar.es/record/60879/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000060879 909CO $$ooai:zaguan.unizar.es:60879$$particulos$$pdriver
000060879 951__ $$a2017-05-03-14:22:49
000060879 980__ $$aARTICLE