000060720 001__ 60720
000060720 005__ 20190819101351.0
000060720 0247_ $$2doi$$a10.1167/iovs.16-21100
000060720 0248_ $$2sideral$$a98197
000060720 037__ $$aART-2017-98197
000060720 041__ $$aeng
000060720 100__ $$0(orcid)0000-0003-2770-4617$$aOrtillés, Á.$$uUniversidad de Zaragoza
000060720 245__ $$aA rabbit model of Acanthamoeba keratitis: Use of infected soft contact lenses after corneal epithelium debridement with a diamond burr
000060720 260__ $$c2017
000060720 5060_ $$aAccess copy available to the general public$$fUnrestricted
000060720 5203_ $$aPURPOSE. To develop a rabbit model of Acanthamoeba keratitis (AK) as the best method to reproduce the natural course of this disease. METHODS. To induce AK, infected contact lenses (1000 amoebae/mm2, 90% trophozoites) were placed over the previously debrided corneal surface, in combination with a temporary tarsorrhaphy. Environmental and clinical strains of Acanthamoeba spp. (genotype T4) were used. Three groups (1L, n = 32; 2L–21d, n = 5; 2L–3d, n = 23) were established according to the number of contact lenses used (1L, 1 lens; 2L–21d and 2L–3d, 2 lenses) and the placement day of these (1L, day 1; 2L–21d, days 1 and 21; 2L–3d, days 1 and 3). The infection was quantified by a clinical score system and confirmed using corneal cytology and culture, polymerase chain reaction and histopathologic analysis. RESULTS. The infection rate obtained was high (1L, 87.5%; 2L–21d, 100%; 2L–3d, 82.6%), although no clinical signs were observed in the 50% of the infected animals in group 1L. Among groups, group 2L–3d showed more cases of moderate and severe infection. Among strains, no statistically significant differences were found in the infection rate. In the control eyes, cross infection was confirmed when a sterile contact lens was placed in the previously debrided corneas but not if the eye remained intact. CONCLUSIONS. The combination of two infected contact lenses after corneal debridement seems to be an alternative model, clinically and histopathologically similar to its human counterpart, to induce the different AK stages and reproduce the course of the disease in rabbits.
000060720 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/DPI2014-54981-R$$9info:eu-repo/grantAgreement/ES/MEC/FPU13-03782$$9info:eu-repo/grantAgreement/ES/DGA/T88$$9info:eu-repo/grantAgreement/ES/DGA/B124
000060720 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000060720 590__ $$a3.388$$b2017
000060720 591__ $$aOPHTHALMOLOGY$$b9 / 59 = 0.153$$c2017$$dQ1$$eT1
000060720 592__ $$a2.058$$b2017
000060720 593__ $$aCellular and Molecular Neuroscience$$c2017$$dQ1
000060720 593__ $$aSensory Systems$$c2017$$dQ1
000060720 593__ $$aOphthalmology$$c2017$$dQ1
000060720 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000060720 700__ $$0(orcid)0000-0003-0765-7227$$aGoñi, P.$$uUniversidad de Zaragoza
000060720 700__ $$0(orcid)0000-0002-9273-5885$$aRubio, E.$$uUniversidad de Zaragoza
000060720 700__ $$0(orcid)0000-0002-6134-8698$$aSierra, M.$$uUniversidad de Zaragoza
000060720 700__ $$aGámez, E.
000060720 700__ $$0(orcid)0000-0002-6455-119X$$aFernández, M.T.$$uUniversidad de Zaragoza
000060720 700__ $$aBenito, M.$$uUniversidad de Zaragoza
000060720 700__ $$aCristóbal, J.Á.
000060720 700__ $$0(orcid)0000-0001-9713-1813$$aCalvo, B.$$uUniversidad de Zaragoza
000060720 7102_ $$11008$$2660$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Parasitología
000060720 7102_ $$11006$$2255$$aUniversidad de Zaragoza$$bDpto. Fisiatría y Enfermería$$cÁrea Enfermería
000060720 7102_ $$11005$$2315$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Farmacología
000060720 7102_ $$11008$$2615$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Medic.Prevent.Salud Públ.
000060720 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000060720 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000060720 7102_ $$15005$$2790$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Tecnologi. Medio Ambiente
000060720 773__ $$g58, 2 (2017), 1218-1227$$pInvestig. ophthalmol. vis. sci.$$tINVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE$$x0146-0404
000060720 8564_ $$s1342387$$uhttps://zaguan.unizar.es/record/60720/files/texto_completo.pdf$$yVersión publicada
000060720 8564_ $$s128074$$uhttps://zaguan.unizar.es/record/60720/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000060720 909CO $$ooai:zaguan.unizar.es:60720$$particulos$$pdriver
000060720 951__ $$a2019-08-19-09:51:17
000060720 980__ $$aARTICLE