000060617 001__ 60617
000060617 005__ 20240202150201.0
000060617 0247_ $$2doi$$a10.3390/chromatography2020167
000060617 0248_ $$2sideral$$a93070
000060617 037__ $$aART-2015-93070
000060617 041__ $$aeng
000060617 100__ $$aDominguez, A
000060617 245__ $$aA Hyphenated Technique based on High-Performance Thin Layer Chromatography for Determining Neutral Sphingolipids: A Proof of concept
000060617 260__ $$c2015
000060617 5060_ $$aAccess copy available to the general public$$fUnrestricted
000060617 5203_ $$aHyphenated HPTLC has been used to analyze several neutral sphingolipids acting as lysosomal storage disease (LSD) biomarkers. Automated multiple development (AMD) provides separation of lipid peaks, which are detected and quantified using fluorescence detection by intensity changes (FDIC) after primuline post-impregnation. A final online transfer to a mass spectrometer by means of an elution-based interface allows their identification using electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI).Given that the increases in fluorescent emission detected by FDIC are produced by non-specific, electrostatic interactions between the primuline and hydrocarbon chains in the ceramide backbones of sphingolipids, it is a non-destructive detection technique, allowing the precise location and transfer of biomarker peaks to a mass spectrometer using an elution interface. By using primuline as a fluorophore, the technique is also compatible with ESI-APCI and does not interfere with the MS of sphingolipids. APCI provides useful and complementary structural information to the ESI for sphingolipid identification. Moreover, FDIC emission can be used for quantitative purposes. Results include the determination of sphingomyelin (SM) in human-plasma samples (RSD < 6%) by means of a standard addition method with non-linear calibration, and the identification of globotriaosylceramide (Gb3) in the plasma of a Fabry patient. Only one HPTLC plate is needed to perform the analysis.
000060617 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2012-035535$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2012-34774
000060617 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000060617 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000060617 700__ $$0(orcid)0000-0002-8701-9745$$aJarne, C
000060617 700__ $$aCebolla, VL
000060617 700__ $$0(orcid)0000-0002-8973-5104$$aGalbán, J$$uUniversidad de Zaragoza
000060617 700__ $$0(orcid)0000-0001-7299-0187$$aSavirón, M
000060617 700__ $$0(orcid)0000-0003-3514-2570$$aOrduna, J$$uUniversidad de Zaragoza
000060617 700__ $$aMembrado, L
000060617 700__ $$aLapieza, MP
000060617 700__ $$aRomero, E
000060617 700__ $$0(orcid)0000-0002-3906-4576$$aSanz-Vicente, I$$uUniversidad de Zaragoza
000060617 700__ $$0(orcid)0000-0002-7902-6005$$ade Marcos, S$$uUniversidad de Zaragoza
000060617 700__ $$0(orcid)0000-0003-2607-7834$$aGarriga, R$$uUniversidad de Zaragoza
000060617 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000060617 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000060617 7102_ $$12012$$2755$$aUniversidad de Zaragoza$$bDpto. Química Física$$cÁrea Química Física
000060617 773__ $$g2, 2 (2015), 167-187$$pChromatography (Basel)$$tChromatography (Basel)$$x2227-9075
000060617 8564_ $$s711608$$uhttps://zaguan.unizar.es/record/60617/files/texto_completo.pdf$$yVersión publicada
000060617 8564_ $$s89870$$uhttps://zaguan.unizar.es/record/60617/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000060617 909CO $$ooai:zaguan.unizar.es:60617$$particulos$$pdriver
000060617 951__ $$a2024-02-02-14:57:26
000060617 980__ $$aARTICLE