000058522 001__ 58522
000058522 005__ 20170327111855.0
000058522 0248_ $$2sideral$$a80240
000058522 037__ $$aART-2013-80240
000058522 041__ $$aeng
000058522 100__ $$aSarasa, Rocío
000058522 245__ $$aA comparative study about confirmatory techniques on non-conclusive Bovine Spongiform Encephalopathy cases
000058522 260__ $$c2013
000058522 5060_ $$aAccess copy available to the general public$$fUnrestricted
000058522 5203_ $$aBackground: In the framework of the Bovine Spongiform Encephalopathy (BSE) surveillance programme, samples with non-conclusive results using the OIE confirmatory techniques have been repeatedly found. It is therefore necessary to question the adequacy of the previously established consequences of this non-conclusive result: the danger of failing to detect potentially infected cattle or erroneous information that may affect the decision of culling or not of an entire bovine cohort. Moreover, there is a very real risk that the underreporting of cases may possibly lead to distortion of the BSE epidemiological information for a given country. In this study, samples from bovine nervous tissue presenting non-conclusive results by conventional OIE techniques (Western blot and immunohistochemistry) were analyzed. Their common characteristic was a very advanced degree of autolysis. All techniques recommended by the OIE for BSE diagnosis were applied on all these samples in order to provide a comparative study. Specifically, immunohistochemistry, Western blotting, SAF detection by electron microscopy and mouse bioassay were compared. Besides, other non confirmatory techniques, confocal scanning microscopy and colloidal gold labelling of fibrils, were applied on these samples for confirming and improving the results. 
Results: Immunocytochemistry showed immunostaining in agreement with the positive results finally provided by the other confirmatory techniques. These results corroborated the suitability of this technique which was previously developed to examine autolysed (liquified) brain samples. Transmission after inoculation of a transgenic murine model TgbovXV was successful in all inocula but not in all mice, perhaps due to the very scarce PrPsc concentration present in samples. Electron microscopy, currently fallen into disuse, was demonstrated to be, not only capable to provide a final diagnosis despite the autolytic state of samples, but also to be a sensitive diagnostic alternative for resolving cases with low concentrations of PrPsc. 
Conclusions: Demonstration of transmission of the disease even with low concentrations of PrPsc should reinforce that vigilance is required in interpreting results so that subtle changes do not go unnoticed. To maintain a continued supervision of the techniques which are applied in the routine diagnosis would prove essential for the ultimate eradication of the disease.
000058522 536__ $$9info:eu-repo/grantAgreement/ES/CICYT/AGL2006-08467
000058522 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000058522 590__ $$a3.383$$b2013
000058522 591__ $$aVETERINARY SCIENCES$$b1 / 129 = 0.008$$c2013$$dQ1$$eT1
000058522 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000058522 700__ $$aBecher, Dietman
000058522 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan José$$uUniversidad de Zaragoza
000058522 700__ $$0(orcid)0000-0002-2787-9671$$aMonzón, Marta$$uUniversidad de Zaragoza
000058522 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDepartamento de Patología Animal$$cSanidad Animal
000058522 773__ $$g9, 212 (2013), [11 pp]$$pVet. res.$$tVETERINARY RESEARCH$$x0928-4249
000058522 8564_ $$s2786511$$uhttp://zaguan.unizar.es/record/58522/files/texto_completo.pdf$$yVersión publicada
000058522 8564_ $$s93696$$uhttp://zaguan.unizar.es/record/58522/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000058522 909CO $$ooai:zaguan.unizar.es:58522$$particulos$$pdriver
000058522 951__ $$a2017-01-31-13:08:46
000058522 980__ $$aARTICLE