000058514 001__ 58514
000058514 005__ 20170327111855.0
000058514 0247_ $$2doi$$a10.1186/1297-9716-45-25
000058514 0248_ $$2sideral$$a85545
000058514 037__ $$aART-2014-85545
000058514 041__ $$aeng
000058514 100__ $$0(orcid)0000-0002-9453-7655$$aDel Cacho, Emilio$$uUniversidad de Zaragoza
000058514 245__ $$aIL-17A regulates Eimeria tenella schizont maturation and migration in avian coccidiosis
000058514 260__ $$c2014
000058514 5060_ $$aAccess copy available to the general public$$fUnrestricted
000058514 5203_ $$aAlthough IL17A is associated with the immunological control of various infectious diseases, its role in host response to Eimeria infections is not well understood. In an effort to better dissect the role of IL17A in host-pathogen interactions in avian coccidiosis, a neutralizing antibody (Ab) to chicken IL17A was used to counteract IL17A bioactivity in vivo. Chickens infected with Eimeria tenella and treated intravenously with IL17A Ab, exhibited reduced intracellular schizont and merozoite development, diminished lesion score, compared with untreated controls. Immunohistological evaluation of cecal lesions in the parasitized tissues indicated reduced migration and maturation of second-generation schizonts and reduced lesions in lamina propria and submucosa. In contrast, untreated and infected chickens had epithelial cells harboring second-generation schizonts, which extend into the submucosa through muscularis mucosa disruptions, maturing into second generation merozoites. Furthermore, IL17A Ab treatment was associated with increased parameters of Th1 immunity (IL2- and IFN¿- producing cells), reduced levels of reactive oxygen species (ROS), and diminished levels of serum matrix metalloproteinase-9 (MMP-9). Finally, schizonts from untreated and infected chickens expressed S100, Wiskott-Aldrich syndrome protein family member 3 (WASF3), and heat shock protein-70 (HSP70) proteins as merozoites matured, whereas the expression of these proteins was absent in IL17A Ab-treated chickens. These results provide the first evidence that the administration of an IL17A neutralizing Ab to E. tenella-infected chickens inhibits the migration of parasitized epithelial cells, markedly reduces the production of ROS and MMP-9, and decreases cecal lesions, suggesting that IL17A might be a potential therapeutic target for coccidiosis control.
000058514 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A46
000058514 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000058514 590__ $$a2.815$$b2014
000058514 591__ $$aVETERINARY SCIENCES$$b3 / 132 = 0.023$$c2014$$dQ1$$eT1
000058514 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000058514 700__ $$0(orcid)0000-0003-3945-4854$$aGallego, Margarita$$uUniversidad de Zaragoza
000058514 700__ $$aLillehoj, Hyun Soon
000058514 700__ $$0(orcid)0000-0001-7831-2483$$aQuílez, Joaquín$$uUniversidad de Zaragoza
000058514 700__ $$aLillehoj, Erik P
000058514 700__ $$aRamo, Ana
000058514 700__ $$aSánchez-Acedo, Caridad
000058514 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDepartamento de Patología Animal$$cSanidad Animal
000058514 773__ $$g45, 25 (2014), 1-9$$pVet. res.$$tVETERINARY RESEARCH$$x0928-4249
000058514 8564_ $$s347686$$uhttps://zaguan.unizar.es/record/58514/files/texto_completo.pdf$$yVersión publicada
000058514 8564_ $$s115928$$uhttps://zaguan.unizar.es/record/58514/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000058514 909CO $$ooai:zaguan.unizar.es:58514$$particulos$$pdriver
000058514 951__ $$a2017-01-17-13:23:15
000058514 980__ $$aARTICLE