000057908 001__ 57908
000057908 005__ 20170327111930.0
000057908 0247_ $$2doi$$a10.1155/2014/287896
000057908 0248_ $$2sideral$$a88580
000057908 037__ $$aART-2014-88580
000057908 041__ $$aeng
000057908 100__ $$aSrivastava, Girish Kumar
000057908 245__ $$aChitosan feasibility to retain retinal stem cell phenotype and slow proliferation for retinal transplantation
000057908 260__ $$c2014
000057908 5060_ $$aAccess copy available to the general public$$fUnrestricted
000057908 5203_ $$aRetinal stem cells (RSCs) are promising in cell replacement strategies for retinal diseases. RSCs can migrate, differentiate, and integrate into retina. However, RSCs transplantation needs an adequate support; chitosan membrane (ChM) could be one, which can carry RSCs with high feasibility to support their integration into retina. RSCs were isolated, evaluated for phenotype, and subsequently grown on sterilized ChM and polystyrene surface for 8 hours, 1, 4, and 11 days for analysing cell adhesion, proliferation, viability, and phenotype. Isolated RSCs expressed GFAP, PKC, isolectin, recoverin, RPE65, PAX-6, cytokeratin 8/18, and nestin proteins. They adhered (28 ± 16%, 8 hours) and proliferated (40 ± 20 cells/field, day 1 and 244 ± 100 cells/field, day 4) significantly low on ChM. However, they maintained similar viability (>95%) and phenotype (cytokeratin 8/18, PAX6, and nestin proteins expression, day 11) on both surfaces (ChM and polystyrene). RSCs did not express alpha-SMA protein on both surfaces. RSCs express proteins belonging to epithelial, glial, and neural cells, confirming that they need further stimulus to reach a final destination of differentiation that could be provided in in vivo condition. ChM does not alternate RSCs behaviour and therefore can be used as a cell carrier so that slow proliferating RSCs can migrate and integrate into retina.
000057908 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/ISCIII-PS09-00938
000057908 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000057908 590__ $$a1.579$$b2014
000057908 591__ $$aMEDICINE, RESEARCH & EXPERIMENTAL$$b85 / 123 = 0.691$$c2014$$dQ3$$eT3
000057908 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b106 / 162 = 0.654$$c2014$$dQ3$$eT2
000057908 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000057908 700__ $$aRodríguez-Crespo, David
000057908 700__ $$aSingh, Amar Kumar
000057908 700__ $$aCasado-Coterillo, Clara
000057908 700__ $$aFernandez-Bueno, Ivan
000057908 700__ $$aGarcia-Gutierrez, Maria T.
000057908 700__ $$0(orcid)0000-0003-1512-4500$$aCoronas, Joaquin$$uUniversidad de Zaragoza
000057908 700__ $$aPastor, J. Carlos
000057908 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDepartamento de Ingeniería Química y Tecnologías del Medio Ambiente$$cIngeniería Química
000057908 773__ $$g2014 (2014), 287896 [10 pp]$$pBioMed res. int.$$tBioMed Research International$$x2314-6133
000057908 8564_ $$s3625269$$uhttps://zaguan.unizar.es/record/57908/files/texto_completo.pdf$$yVersión publicada
000057908 8564_ $$s101680$$uhttps://zaguan.unizar.es/record/57908/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000057908 909CO $$ooai:zaguan.unizar.es:57908$$particulos$$pdriver
000057908 951__ $$a2016-12-16-14:53:32
000057908 980__ $$aARTICLE