000057752 001__ 57752 000057752 005__ 20210121114545.0 000057752 0247_ $$2doi$$a10.1111/andr.12054 000057752 0248_ $$2sideral$$a91704 000057752 037__ $$aART-2015-91704 000057752 041__ $$aeng 000057752 100__ $$aYeste, M. 000057752 245__ $$aIntracellular calcium movements of boar spermatozoa during 'in vitro' capacitation and subsequent acrosome exocytosis follow a multiple-storage place, extracellular calcium-dependent model 000057752 260__ $$c2015 000057752 5060_ $$aAccess copy available to the general public$$fUnrestricted 000057752 5203_ $$aThis work analysed intracellular calcium stores of boar spermatozoa subjected to invitro' capacitation (IVC) and subsequent progesterone-induced acrosome exocytosis (IVAE). Intracellular calcium was analysed through two calcium markers with different physico-chemical properties, Fluo-3 and Rhod-5N. Indicative parameters of IVC and IVAE were also evaluated. Fluo-3 was located at both the midpiece and the whole head. Rhod-5N was present at the sperm head. This distribution did not change in any of the assayed conditions. Induction of IVC was concomitant with an increase in both head and midpiece Ca2+ signals. Additionally, while IVC induction was concurrent with a significant (p<0.05) increase in sperm membrane permeability, no significant changes were observed in O-2 consumption and ATP levels. Incubation of boar spermatozoa in the absence of calcium showed a loss of both Ca2+ labellings concomitantly with the sperm's inability to achieve IVC. The absence of extracellular calcium also induced a severe decrease in the percentage of spermatozoa exhibiting high mitochondrial membrane potential (hMMP). The IVAE was accompanied by a fast increase in both Ca2+ signalling in control spermatozoa. These peaks were either not detected or much lessened in the absence of calcium. Remarkably, Fluo-3 marking at the midpiece increased after progesterone addition to sperm cells incubated in a medium without Ca2+. The simultaneous addition of progesterone with the calcium chelant EGTA inhibited IVAE, and this was accompanied by a significant (p<0.05) decrease in the intensity of progesterone Ca2+-induced peak, O-2 consumption and ATP levels. Our results suggest that boar spermatozoa present different calcium deposits with a dynamic equilibrium among them and with the extracellular environment. Additionally, the modulation role of the intracellular calcium in spermatozoa function seems to rely on its precise localization in boar spermatozoa. 000057752 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/JCI-2010-08620$$9info:eu-repo/grantAgreement/EC/FP7/626061/EU/Oocyte activation and human infertility: Identification and characterisation of a critical oocyte-borne receptor, and effects of laboratory procedures upon key proteins involved in oocyte activation/OOCYTE ACTIVATION 000057752 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/ 000057752 590__ $$a2.515$$b2015 000057752 591__ $$aANDROLOGY$$b2 / 5 = 0.4$$c2015$$dQ2$$eT2 000057752 592__ $$a1.015$$b2015 000057752 593__ $$aReproductive Medicine$$c2015$$dQ1 000057752 593__ $$aUrology$$c2015$$dQ1 000057752 593__ $$aEndocrinology$$c2015$$dQ2 000057752 593__ $$aEndocrinology, Diabetes and Metabolism$$c2015$$dQ2 000057752 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/submittedVersion 000057752 700__ $$aFernández-Novell, J.M. 000057752 700__ $$aRamió-Lluch, L. 000057752 700__ $$aEstrada, E. 000057752 700__ $$aRocha, L.G. 000057752 700__ $$0(orcid)0000-0001-9842-6197$$aCebrián-Pérez, J.A.$$uUniversidad de Zaragoza 000057752 700__ $$0(orcid)0000-0003-1306-8517$$aMuiño-Blanco, T.$$uUniversidad de Zaragoza 000057752 700__ $$aConcha, I.I. 000057752 700__ $$aRamírez, A. 000057752 700__ $$aRodríguez-Gil, J.E. 000057752 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología 000057752 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole. 000057752 773__ $$g3, 4 (2015), 729-747$$pAndrology$$tAndrology$$x2047-2919 000057752 8564_ $$s1109130$$uhttps://zaguan.unizar.es/record/57752/files/texto_completo.pdf$$yPreprint 000057752 8564_ $$s55312$$uhttps://zaguan.unizar.es/record/57752/files/texto_completo.jpg?subformat=icon$$xicon$$yPreprint 000057752 909CO $$ooai:zaguan.unizar.es:57752$$particulos$$pdriver 000057752 951__ $$a2021-01-21-11:18:36 000057752 980__ $$aARTICLE