Characterization of mesenchymal stem cells in sheep naturally infected with scrapie

Mediano, Diego R. ; Sanz-Rubio, David ; Bolea, Rosa (Universidad de Zaragoza) ; Marín, Belén ; Vázquez, Francisco J. (Universidad de Zaragoza) ; Remacha, Ana R. (Universidad de Zaragoza) ; López-Pérez, Óscar (Universidad de Zaragoza) ; Fernández-Borges, Natalia ; Castilla, Joaquín ; Zaragoza, Pilar (Universidad de Zaragoza) ; Badiola, Juan J. (Universidad de Zaragoza) ; Rodellar, Clementina (Universidad de Zaragoza) ; Martín-Burriel, Inmaculada (Universidad de Zaragoza)
Characterization of mesenchymal stem cells in sheep naturally infected with scrapie
Resumen: Mesenchymal stem cells (MSCs) can be infected with prions and have been proposed as in vitro cell-based models for prion replication. In addition, autologous MSCs are of interest for cell therapy in neurodegenerative diseases. To the best of our knowledge, the effect of prion diseases on the characteristics of these cells has never been investigated. Here, we analysed the properties of MSCs obtained from bone marrow (BM-MSCs) and peripheral blood (PB-MSCs) of sheep naturally infected with scrapie — a large mammal model for the study of prion diseases. After three passages of expansion, MSCs derived from scrapie animals displayed similar adipogenic, chondrogenic and osteogenic differentiation ability as cells from healthy controls, although a subtle decrease in the proliferation potential was observed. Exceptionally, mesenchymal markers such as CD29 were significantly upregulated at the transcript level compared with controls. Scrapie MSCs were able to transdifferentiate into neuron-like cells, but displayed lower levels of neurogenic markers at basal conditions, which could limit this potential. The expression levels of cellular prion protein (PrPC) were highly variable between cultures, and no significant differences were observed between control and scrapie-derived MSCs. However, during neurogenic differentiation the expression of PrPC was upregulated in MSCs. This characteristic could be useful for developing in vitro models for prion replication. Despite the infectivity reported for MSCs obtained from scrapie-infected mice and Creutzfeldt–Jakob disease patients, protein misfolding cyclic amplification did not detect PrPSc in BM- or PB-MSCs from scrapie-infected sheep, which limits their use for in vivo diagnosis for scrapie.
Idioma: Inglés
DOI: 10.1099/jgv.0.000292
Año: 2015
Publicado en: JOURNAL OF GENERAL VIROLOGY 96 (2015), 3715-3726
ISSN: 0022-1317

Factor impacto: 3.192 (2015)
Categ. JCR: VIROLOGY rank: 13 / 33 = 0.394 (2015) - Q2 - T2
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 46 / 161 = 0.286 (2015) - Q2 - T1

Financiación: info:eu-repo/grantAgreement/ES/DGA/CTPP2-13
Financiación: info:eu-repo/grantAgreement/ES/DGA/CTP2013-P05
Financiación: info:eu-repo/grantAgreement/ES/UZ/2012-BIO-03
Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Sanidad Animal (Departamento de Patología Animal)
Área (Departamento): Genética (Departamento de Anatomía, Embriología y Genética Animal)
Área (Departamento): Medicina y Cirugía Animal (Departamento de Patología Animal)


Creative Commons Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. No puede utilizar el material para una finalidad comercial. Si remezcla, transforma o crea a partir del material, no puede difundir el material modificado.


Exportado de SIDERAL (2018-02-22-09:47:15)

Este artículo se encuentra en las siguientes colecciones:
Artículos > Artículos por área > Medicina y Cirugía Animal
Artículos > Artículos por área > Sanidad Animal
Artículos > Artículos por área > Genética



 Registro creado el 2016-03-07, última modificación el 2018-02-22


Versión publicada:
 PDF
Valore este documento:

Rate this document:
1
2
3
 
(Sin ninguna reseña)