000032759 001__ 32759
000032759 005__ 20210121114527.0
000032759 0247_ $$2doi$$a10.1371/journal.pone.0137008
000032759 0248_ $$2sideral$$a92964
000032759 037__ $$aART-2015-92964
000032759 041__ $$aeng
000032759 100__ $$0(orcid)0000-0001-5276-7834$$aSerrano, E.$$uUniversidad de Zaragoza
000032759 245__ $$aNew insights into the phylogeny and gene context analysis of binder of sperm proteins (BSPs)
000032759 260__ $$c2015
000032759 5060_ $$aAccess copy available to the general public$$fUnrestricted
000032759 5203_ $$aSeminal plasma (SP) proteins support the survival of spermatozoa acting not only at the plasma membrane but also by inhibition of capacitation, resulting in higher fertilizing ability. Among SP proteins, BSP (binder of sperm) proteins are the most studied, since they may be useful for the improvement of semen diluents, storage and subsequent fertilization results. However, an updated and detailed phylogenetic analysis of the BSP protein superfamily has not been carried out with all the sequences described in the main databases. The update view shows for the first time an equally distributed number of sequences between the three families: BSP, and their homologs 1 (BSPH1) and 2 (BSPH2). The BSP family is divided in four subfamilies, BSP1 subfamily being the predominant, followed by subfamilies BSP3, BSP5 and BSP2. BSPH proteins were found among placental mammals (Eutheria) belonging to the orders Proboscidea, Primates, Lagomorpha, Rodentia, Chiroptera, Perissodactyla and Cetartiodactyla. However, BSPH2 proteins were also found in the Scandentia order and Metatheria clade. This phylogenetic analysis, when combined with a gene context analysis, showed a completely new evolutionary scenario for the BSP superfamily of proteins with three defined different gene patterns, one for BSPs, one for BSPH1/ BSPH2/ELSPBP1 and another one for BSPH1/BSPH2 without ELSPBP1. In addition, the study has permitted to define concise conserved blocks for each family (BSP, BSPH1 and BSPH2), which could be used for a more reliable assignment for the incoming sequences, for data curation of current databases, and for cloning new BSPs, as the one described in this paper, ram seminal vesicle 20 kDa protein (RSVP20, Ovis aries BSP5b).
000032759 536__ $$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/BIO2013-45336-R$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/BIO2010-22225-C02-01$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL2013-43328-P$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL2011–25850$$9info:eu-repo/grantAgreement/ES/DGA/26FSE
000032759 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000032759 590__ $$a3.057$$b2015
000032759 591__ $$aMULTIDISCIPLINARY SCIENCES$$b11 / 62 = 0.177$$c2015$$dQ1$$eT1
000032759 592__ $$a1.427$$b2015
000032759 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2015$$dQ1
000032759 593__ $$aMedicine (miscellaneous)$$c2015$$dQ1
000032759 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2015$$dQ1
000032759 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000032759 700__ $$aMartínez, A.B.
000032759 700__ $$aArruga, D.
000032759 700__ $$0(orcid)0000-0002-2312-6402$$aPérez-Pé, R.$$uUniversidad de Zaragoza
000032759 700__ $$aSánchez-Ferrer, Á.
000032759 700__ $$0(orcid)0000-0003-1306-8517$$aMuiño-Blanco, T.$$uUniversidad de Zaragoza
000032759 700__ $$0(orcid)0000-0001-9842-6197$$aCebrián-Pérez, J.A.$$uUniversidad de Zaragoza
000032759 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000032759 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000032759 773__ $$g10, 9 (2015), [19 pp]$$pPLoS One$$tPloS one$$x1932-6203
000032759 8564_ $$s9047057$$uhttps://zaguan.unizar.es/record/32759/files/texto_completo.pdf$$yVersión publicada
000032759 8564_ $$s102023$$uhttps://zaguan.unizar.es/record/32759/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000032759 909CO $$ooai:zaguan.unizar.es:32759$$particulos$$pdriver
000032759 951__ $$a2021-01-21-11:07:20
000032759 980__ $$aARTICLE