Repositorio Institucional de Documentos

Resumen: Background: Antimicrobial photodynamic therapy has been proposed as an alternative to suppress subgingival species. This results from the balance among Streptococcus sanguis, Streptococcus mutans and Candida albicans in the dental biofilm. Not all the photosensitizers have the same photodynamic effect against the different microorganims. The objective of this study is to compare in vitro the photodynamic effect of methylene blue (MB), rose Bengal (RB) and curcumin (CUR) in combination with white light on the cariogenic microorganism S. mutans, S. sanguis and C. albicans. Results: Photodynamic therapy with MB, RB and CUR inhibited 6 log 10 the growth of both bacteria but at different concentrations: 0.31-0.62 µg/ml and 0.62-1.25 µg/ml RB were needed to photoinactivate S. mutans and S. sanguis, respectively//1.25-2.5 µg/ml MB for both species//whereas higher CUR concentrations (80-160 µg/ml and 160-320 µg/ml) were required to obtain the same reduction in S. mutans and S. sanguis viability respectively. The minimal fungicidal concentration of MB for 5 log10 CFU reduction (4.5 McFarland) was 80-160 µg/ml, whereas for RB it ranged between 320 and 640 µg/ml. For CUR, even the maximum studied concentration (1280 µg/ml) did not reach that inhibition. Incubation time had no effect in all experiments. Conclusions: Photodynamic therapy with RB, MB and CUR and white light is effective in killing S. mutans and S. sanguis strains, although MB and RB are more efficient than CUR. C. albicans required higher concentrations of all photosensitizers to obtain a fungicidal effect, being MB the most efficient and CUR ineffective.
Idioma: Inglés
DOI: 10.1186/s12866-015-0524-3
Año: 2015
Publicado en: BMC MICROBIOLOGY 15, 187 (2015), [8 pp.]
ISSN: 1471-2180
Factor impacto JCR: 2.581 (2015)
Categ. JCR: MICROBIOLOGY rank: 58 / 123 = 0.472 (2015) - Q2 - T2
Factor impacto SCIMAGO: 1.42 - Microbiology (medical) (Q1) - Microbiology (Q2)

Financiación: info:eu-repo/grantAgreement/ES/MICINN/CTQ2013-48767-C3-2-R
Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Área Microbiología (Dpto. Microb.Med.Pr.,Sal.Públ.)
Área (Departamento): Área Óptica (Dpto. Física Aplicada)

Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. No puede utilizar el material para una finalidad comercial. Si remezcla, transforma o crea a partir del material, no puede difundir el material modificado.

Exportado de SIDERAL (2021-01-21-08:15:24)


Visitas y descargas

Este artículo se encuentra en las siguientes colecciones:
Artículos